{Reference Type}: Journal Article
{Author}: Inceoglu, Ozgul; van Overbeek, Leo Simon; Salles, Joana Falcao; van Elsas, Jan Dirk
{Year}: 2013
{Title}: Normal operating range of bacterial communities in soil used for potato cropping
{Tag}: 0
{Star}: 0
{Place Published}: 1752 N Street N.W., Washington, DC 20036-2904, United States
{Journal}: Applied and Environmental Microbiology
{Volume}: 79
{Issue}: 4
{Pages}: 1160-1170
{Date Displayed}: 2013
{ISBN/ISSN}: 00992240
{Original Publication}: American Society for Microbiology
{Keywords}: Bacteria; Cultivation; Electrophoresis; Plants (botany); Polymerase chain reaction; Social sciences; Soils; Starch; Tubes (components)
{Abstract}: In this study, the impacts of six potato (Solanum tuberosum) cultivars with different tuber starch allocations (including one genetically modified [GM] line) on the bacterial communities in field soil were investigated across two growth seasons interspersed with 1year of barley cultivation, using quantitative PCR, clone library, and PCR-denaturing gradient gel electrophoresis (DGGE) analyses. It was hypothesized that the modifications in the tuber starch contents of these plants, yielding changed root growth rates and exudation patterns, might have elicited altered bacterial communities in the soil. The data showed that bacterial abundances in the bulk soil varied over about 2 orders of magnitude across the 3 years. As expected, across all cultivars, positive potato rhizosphere effects on bacterial abundances were noted in the two potato years. The bulk soil bacterial community structures revealed progressive shifts across time, and moving-window analysis revealed a 60% change over the total experiment. Consistent with previous findings, the community structures in the potato rhizosphere compartments were mainly affected by the growth stage of the plants and, to a lesser extent, by plant cultivar type. The data from the soil under the non-GM potato lines were then taken to define the normal operating range (NOR) of the microbiota under potatoes. Interestingly, the bacterial communities under the GM potato line remained within this NOR. In regard to the bacterial community compositions, particular bacterial species in the soil appeared to be specific to (i) the plant species under investigation (barley versus potato) or, with respect to potatoes, (ii) the plant growth stage. Members of the genera Arthrobacter, Streptomyces, Rhodanobacter, and Dokdonella were consistently found only at the flowering potato plants in both seasons, whereas Rhodoplanes and Sporosarcina were observed only in the soil planted to barley.   2013, American Society for Microbiology.
{Notes}: Compilation and indexing terms, Copyright 2013 Elsevier Inc.
20131116116239
Across time
Arthrobacter
Bacterial abundance
Bacterial community
Bacterial community composition
Bacterial species
Bulk soils
Clone library
Community structures
Genetically modified
Gradient gel electrophoresis
Growth season
Growth stages
In-field
Microbiotas
Moving-window
Operating ranges
Orders of magnitude
Plant cultivars
Plant growth stages
Plant species
Potato plants
Quantitative PCR
Rhizosphere effects
Solanum tuberosum
Tuber starch
{Author Address}: Department of Microbial Ecology, Centre for Life Sciences, Groningen, Netherlands

{Reference Type}: Journal Article
{Author}: Linde, C. C.; Selmes, H.
{Year}: 2012
{Title}: Genetic diversity and mating type distribution of tuber melanosporum and their significance to truffle cultivation in artificially planted truffieres in Australia
{Tag}: 0
{Star}: 0
{Place Published}: 1752 N Street N.W., Washington, DC 20036-2904, United States
{Journal}: Applied and Environmental Microbiology
{Volume}: 78
{Issue}: 18
{Pages}: 6534-6539
{Date Displayed}: 2012
{ISBN/ISSN}: 00992240
{Original Publication}: American Society for Microbiology
{Keywords}: Forestry; Biodiversity; Competition; Tubes (components)
{Abstract}: Tuber melanosporum is a truffle native to Europe and is cultivated in countries such as Australia for the gastronomic market, where production yields are often lower than expected. We assessed the genetic diversity of T. melanosporum with six microsatellite loci to assess the effect of genetic drift on truffle yield in Australia. Genetic diversity as assessed on 210 ascocarps revealed a higher allelic diversity compared to previous studies from Europe, suggesting a possible genetic expansion and/or multiple and diverse source populations for inoculum. The results also suggest that the single sequence repeat diversity of locus ME2 is adaptive and that, for example, the probability of replication errors is increased for this locus. Loss of genetic diversity in Australian populations is therefore not a likely factor in limiting ascocarp production. A survey of nursery seedlings and trees inoculated with T. melanosporum revealed that 70% of seedlings and host trees were colonized with T. melanosporum and that some trees had been contaminated by Tuber brumale, presumably during the inoculation process. Mating type (MAT1-1-1 and MAT1-2-1) analyses on seedling and four- to ten-year-old host trees found that 100% of seedlings but only approximately half of host trees had both mating types present. Furthermore, MAT1-1-1 was detected significantly more commonly than MAT1-2-1 in established trees, suggesting a competitive advantage for MAT1-1-1 strains. This study clearly shows that there are more factors involved in ascocarp production than just the presence of both mating types on host trees.   2012, American Society for Microbiology.
{Notes}: Compilation and indexing terms, Copyright 2013 Elsevier Inc.
20123815449452
Allelic diversity
Australia
Competitive advantage
Genetic diversity
Genetic drifts
Inoculation process
Microsatellite loci
Production yield
Single sequence repeats
Source population
Tuber melanosporum
{Author Address}: Evolution, Ecology, and Genetics, Research School of Biology, Australian National University, ACT, Australian Capital Territory, Australia

{Reference Type}: Journal Article
{Author}: Linde, C. C.; Selmes, H.
{Year}: 2012
{Title}: Genetic Diversity and Mating Type Distribution of Tuber melanosporum and Their Significance to Truffle Cultivation in Artificially Planted Truffieres in Australia
{Tag}: 0
{Star}: 0
{Journal}: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
{Volume}: 78
{Issue}: 18
{Pages}: 6534-6539
{ISBN/ISSN}: 0099-2240
{Keywords}: BLACK TRUFFLE; SIMPLE SEQUENCES; DNA; POPULATIONS; ECTOMYCORRHIZAE; OUTCROSSES; EVOLUTION; DYNAMICS; MARKERS; FIELD
{Abstract}: Tuber melanosporum is a truffle native to Europe and is cultivated in countries such as Australia for the gastronomic market, where production yields are often lower than expected. We assessed the genetic diversity of T. melanosporum with six microsatellite loci to assess the effect of genetic drift on truffle yield in Australia. Genetic diversity as assessed on 210 ascocarps revealed a higher allelic diversity compared to previous studies from Europe, suggesting a possible genetic expansion and/or multiple and diverse source populations for inoculum. The results also suggest that the single sequence repeat diversity of locus ME2 is adaptive and that, for example, the probability of replication errors is increased for this locus. Loss of genetic diversity in Australian populations is therefore not a likely factor in limiting ascocarp production. A survey of nursery seedlings and trees inoculated with T. melanosporum revealed that <70% of seedlings and host trees were colonized with T. melanosporum and that some trees had been contaminated by Tuber brumale, presumably during the inoculation process. Mating type (MAT1-1-1 and MAT1-2-1) analyses on seedling and four- to ten-year-old host trees found that 100% of seedlings but only approximately half of host trees had both mating types present. Furthermore, MAT1-1-1 was detected significantly more commonly than MAT1-2-1 in established trees, suggesting a competitive advantage for MAT1-1-1 strains. This study clearly shows that there are more factors involved in ascocarp production than just the presence of both mating types on host trees.
{Author Address}: Australian Natl Univ, Evolut Ecol & Genet Res Sch Biol, Canberra, ACT, Australia; Australian Natl Univ, Evolut Ecol & Genet Res Sch Biol, Canberra, ACT, Australia
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Australia

{Reference Type}: Journal Article
{Author}: Visnovsky, Sandra B.; Guerin-Laguette, Alexis; Wang, Yun; Pitman, Andrew R.
{Year}: 2010
{Title}: Traceability of Marketable Japanese Shoro in New Zealand: Using Multiplex PCR To Exploit Phylogeographic Variation among Taxa in the Rhizopogon Subgenus Roseoli
{Tag}: 0
{Star}: 0
{Journal}: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
{Volume}: 76
{Issue}: 1
{Pages}: 294-302
{ISBN/ISSN}: 0099-2240
{Keywords}: ECTOMYCORRHIZAL FUNGI; HYPOGEOUS FUNGI; PINUS; IDENTIFICATION; SEEDLINGS; TUBER; PRIMERS; COMMUNITIES; CULTIVATION; MUSHROOMS
{Abstract}: Rhizopogon roseolus Corda (synonym Rhizopogon rubescens Tul.), an economically important edible mushroom associated with the Pinaceae (mostly Pinus sp.), has a global distribution resulting from the introduction of exotic trees into the Southern Hemisphere for plantation forestry. However, the marketability of R. roseolus varies with the place of origin. R. roseolus strains cultivated in New Zealand from local carpophores for the Japanese market are morphologically and biologically distinct from those produced in Japan and are consequently considered less valuable. In this study, the ITS1-5.8S-ITS2 rRNA (internal transcribed spacer [ITS]) region was used to examine the phylogenetic relationships of R. roseolus and other closely related fungi belonging to Rhizopogon subgenus Roseoli to determine the genetic basis for phenotypic differences among R. roseolus isolates from different geographic regions. Phylogenetic comparison revealed phylogeographic variation within Rhizopogon subgenus Roseoli. Collections from the United States and Europe grouped into four distinct clades. Rhizopogon roseolus isolates found in New Zealand were closely related to those from the United States, likely due to introduction of Pinus radiata from its native California in the United States. In contrast, Japanese R. roseolus isolates clustered closely with European collections. Phylogenetic differences between Japanese and New Zealand R. roseolus isolates may explain the morphological and biological properties attributed to these geographical variants. The ITS region was subsequently used to design a multiplex PCR for the simultaneous identification of Japanese and New Zealand R. roseolus isolates to track the establishment of ectomycorrhiza on P. radiata seedlings inoculated with commercially valuable R. roseolus. This diagnostic demonstrated the first fruiting of Japanese shoro cultivated on P. radiata in the Southern Hemisphere.
{Author Address}: New Zealand Inst Plant & Food Res Ltd, Christchurch, New Zealand; New Zealand Inst Plant & Food Res Ltd, Christchurch, New Zealand; New Zealand Inst Plant & Food Res Ltd, Christchurch, New Zealand; New Zealand Inst Plant & Food Res Ltd, Christchurch, New Zealand; Lincoln Univ, Bioprotect Res Ctr, Canterbury, New Zealand
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: New Zealand; New Zealand