{Reference Type}: Journal Article
{Author}: Zhou, Bo; Le Jia; Meng, Fanyun; Song, Zhen; Liu, Xiaonan; Deng, Peng; Fan, Keming
{Year}: 2010
{Title}: Statistical optimization of cultivation conditions for exopolysacchride production and mycelia growth by Stropharia rugosoannulata - Springer
{Tag}: 0
{Star}: 0
{Journal}: Annals of Microbiology
{Volume}: Volume 60
{Issue}: Issue 1
{Pages}: pp 89-96
{DOI}: 10.1007/s13213-009-0006-7
{Date}: 2010-03-01
{Abstract}: Response surface methodology (RSM) was successfully applied to investigate the effect of cultivation temperature, time and volume of media on mycelia growth, in the light of exopolysaccharide content (EPC) and dry cell weight (DCW), by the fungus <em class="a-plus-plus">Stropharia rugosoannulata</em> in submerged culture. The three significant factors influencing EPC and DCW were based on the results of a previous Plackett–Burman (PB) design. Results showed that the maximum predicted values of EPC and DCW reached 1,498.17?mg?l<sup class="a-plus-plus">?1</sup> and 9.89?g?l<sup class="a-plus-plus">?1</sup>, while the optimized conditions of cultivation temperature, time and volume of media for EPC and DCW were 28.35°C, 6.67?days, and 110.29?ml, and 28.23°C, 6.58?days, and 109.17?ml, respectively. When the above conditions were 28.29°C, 6.63?days, and 109.69?ml, the simultaneously maximal yield of exopolysaccharide and mycelia biomass were 1,498.08?mg?l<sup class="a-plus-plus">?1</sup> and 9.88?g?l<sup class="a-plus-plus">?1</sup>, respectively. These predicted values were also verified by validation experiments.
{Subject}: Applied Microbiology;Medical Microbiology;Fungus Genetics;Microbial Ecology;Microbial Genetics and Genomics;Microbiology

{Reference Type}: Journal Article
{Author}: Singh, Ajay Veer; Sharma, Abhinay; Johri, Bhavdish N.
{Year}: 2012
{Title}: Phylogenetic profiling of culturable bacteria associated with early phase of mushroom composting assessed by amplified rDNA restriction analysis
{Tag}: 0
{Star}: 0
{Journal}: ANNALS OF MICROBIOLOGY
{Volume}: 62
{Issue}: 2
{Pages}: 675-682
{ISBN/ISSN}: 1590-4261
{Keywords}: AGARICUS-BISPORUS; IDENTIFICATION; CULTIVATION; FUNGI; Mushroom composting; Agaricus bisporus; ARDRA; 16S rDNA; Phylogenetic profiling
{Abstract}: The edible mushroom Agaricus bisporus is grown commercially on composted manure/straw mixtures. Mushroom composting is a fermentation process in which various groups of microorganisms play important roles at different stages of composting. The present study was conducted to explore the mesophilic bacterial diversity in the early phase of mushroom composting. Morphologically all the isolated bacteria were either Gram-positive rods, cocci or Gram-negative rods. The functional diversity of the bacterial isolates was examined by plate enzyme assays, siderophore production and antagonistic property. Good enzymatic activity for amylase, cellulase, xylanase and protease was reported for different bacterial isolates. Nine bacterial isolates showed siderophore production activity. During antibiosis assay, most of the isolates inhibited growth of and . However, a consortium of selected bacterial isolates produced good amounts of lytic enzymes (amylase, cellulase, xylanase and protease) in solid state fermentation experiments that might help in enhancing the composting process. Amplified 16S-rDNA restriction analysis (ARDRA) of bacterial isolates indicated that four groups of nine bacterial isolates had 100% similarity in all the restriction profiles. However, other isolates exhibited discriminatory relationships with each other. The present study reveals culturable mesophilic bacterial diversity and community succession in the early phase of mushroom composting process as well as emphasizing the application of a bacterial consortium to enhance the composting process.
{Author Address}: Govind Ballabh Pant Univ Agr &amp; Technol, Ranichauri 249199, Uttarakhand, India; Jawaharlal Nehru Univ, Sch Life Sci, New Delhi 110067, India; Barkatullah Univ, Dept Biotechnol, Bhopal 462026, India
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: India; India; India

{Reference Type}: Journal Article
{Author}: Naraian, Ram; Srivastava, Jatin; Garg, Satyendra K.
{Year}: 2011
{Title}: Influence of dairy spent wash (DSW) on different cultivation phases and yield response of two Pleurotus mushrooms
{Tag}: 0
{Star}: 0
{Journal}: ANNALS OF MICROBIOLOGY
{Volume}: 61
{Issue}: 4
{Pages}: 853-862
{ISBN/ISSN}: 1590-4261
{Keywords}: SOLID-STATE FERMENTATION; OYSTER MUSHROOM; WHEAT-STRAW; BIOLOGICAL EFFICIENCY; OSTREATUS STRAINS; SUBSTRATE; EFFLUENT; WATER; WASTES; FUNGI; Dairy spent wash; Pleurotus spp.; Biological efficiency; Fruit body yield; Ergosterol
{Abstract}: Two commercial oyster mushroom species, Pleurotus florida PF05 (PF) and Pleurotus sajor-caju PS08 (PS), were cultivated on wheat straw (WS), corn cob (CC) and combination of both (CC+WS) as basal substrates. These substrates were deliberately supplemented with 5, 10, 20 and 40% of dairy spent wash (DSW) as additive. The influence of DSW on cultivation phases of both mushrooms' lifecycle and other parameters such as mycelial growth rate, ergosterol production, mycelial run, pin head formation, yield, biological efficiency (BE) and protein content in fruitbodies was evaluated. Different DSW concentrations significantly (P&lt;0.05) exhibited continuous variations over different parameters. In contrast to the response of spent wash on PS, PF was superior in every phase and parameter. The lower concentrations up to a certain level unexpectedly influenced the cultivation phases of both mushrooms. The fastest growth rate (6.6 mm day(-1)) and highest ergosterol (437.00 mu g g(-1)) were registered at 20% DSW with the combination of CC+WS as basal substrate. The earliest mycelial run and pin head formation by PF were recorded within 17 and 21 days at 10% supplementation of WS substrate. The maximum 2,173.62 g fruit body yield and 108.68% BE were obtained from PF at 10% DSW supplementation to CC+WS substrates. In addition, the highest content of protein (32.9 mg g(-1)) was achieved in the set of CC+WS with 20% DSW added. The results for each parameter of cultivation in supplemented sets were more enhanced compared to unsupplemented sets. The progression of yield response and biological efficiency was noted as flush-I&gt;flush-II&gt;flush-III with considerable differences. These results suggest the use of DSW at low levels for increasing fruit body yield and BE of Pleurotus mushrooms.
{Author Address}: Chhatrapati Shahu Ji Maharaj Univ, Dept Microbiol, Inst Biosci &amp; Biotechnol, Kanpur 208024, Uttar Pradesh, India; Veer Bahadur Singh Purvanchal Univ, Dept Biotechnol, Fac Sci, Jaunpur 222001, UP, India; Chhatrapati Shahu Ji Maharaj Univ, Dept Environm Sci, Inst Biosci &amp; Biotechnol, Kanpur 208024, Uttar Pradesh, India; Dr Ram Manohar Lohia Avadh Univ, Dept Microbiol, Faizabad 224001, Uttar Pradesh, India
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: India; India; India; India

{Reference Type}: Journal Article
{Author}: Zhou, Bo; Jia, Le; Meng, Fanyun; Song, Zhen; Liu, Xiaonan; Deng, Peng; Fan, Keming
{Year}: 2010
{Title}: Statistical optimization of cultivation conditions for exopolysacchride production and mycelia growth by Stropharia rugosoannulata
{Tag}: 0
{Star}: 0
{Journal}: ANNALS OF MICROBIOLOGY
{Volume}: 60
{Issue}: 1
{Pages}: 89-96
{ISBN/ISSN}: 1590-4261
{Keywords}: SUBMERGED CULTURE; BIOMASS; Stropharia rugosoannulata; Biomass; Exopolysaccharide; Optimization; Response surface methodology
{Abstract}: Response surface methodology (RSM) was successfully applied to investigate the effect of cultivation temperature, time and volume of media on mycelia growth, in the light of exopolysaccharide content (EPC) and dry cell weight (DCW), by the fungus Stropharia rugosoannulata in submerged culture. The three significant factors influencing EPC and DCW were based on the results of a previous Plackett-Burman (PB) design. Results showed that the maximum predicted values of EPC and DCW reached 1,498.17 mg l(-1) and 9.89 g l(-1), while the optimized conditions of cultivation temperature, time and volume of media for EPC and DCW were 28.35 degrees C, 6.67 days, and 110.29 ml, and 28.23 degrees C, 6.58 days, and 109.17 ml, respectively. When the above conditions were 28.29 degrees C, 6.63 days, and 109.69 ml, the simultaneously maximal yield of exopolysaccharide and mycelia biomass were 1,498.08 mg l(-1) and 9.88 g l(-1), respectively. These predicted values were also verified by validation experiments.
{Author Address}: Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China; Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China; Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China; Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China; Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China; Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China; Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China