{Reference Type}: Journal Article
{Author}: Chiejina, Nneka V.; Olufokunbi, Joseph Olumide
{Year}: 2010
{Title}: Effects of different substrates on the yield and protein content of Pleurotus tuberregium
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 9
{Issue}: 11
{Pages}: 1573-1577
{ISBN/ISSN}: 1684-5315
{Keywords}: OIL POLLUTED SOIL; WHITE-ROT FUNGUS; TUBER-REGIUM; EDIBLE MUSHROOM; NIGERIA; BIOREMEDIATION; CULTIVATION; Mushroom; Pleurotus tuberregium; substrates; protein
{Abstract}: The effects of seven substrates for the cultivation, yield and protein content of the mushroom, Pleurotus tuberregium (Fries) Singer were investigated. The experimental design used was completely randomized design (CRD) of 7 treatments and 10 replicates. The highest fresh weight yield was obtained from mushrooms grown in river sand and sawdust mixture (T6) while the least was from those grown in top soil mixed with sawdust (T4). Oil palm fruit fibre (OPFF) substrate (T3) produced no fruit bodies at all. Protein analysis of the mushrooms revealed that those grown in river sand alone (T1) had the highest protein content while fruit bodies harvested from sawdust substrate alone (T2) had the least protein content. River sand plus sawdust substrate (T6) is recommended as the best substrate for the production of P. tuberregium in view of the fact that it produced the highest fruit body weight and its protein content was not significantly lower than that of river sand alone (T2).
{Author Address}: Univ Nigeria, Dept Bot, Nsukka, Nigeria; Univ Nigeria, Dept Bot, Nsukka, Nigeria
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Nigeria

{Reference Type}: Journal Article
{Author}: Wang, Xiqin; Zhang, Jianqiang; Liu, Benhong; Qiu, Dunlian
{Year}: 2010
{Title}: Spent bleaching clay (SBC) from oil refining as a substrate for the spawn production of shiitake mushroom (Lentinula edodes)
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 9
{Issue}: 53
{Pages}: 9007-9011
{ISBN/ISSN}: 1684-5315
{Keywords}: CULTIVATION; FUEL; Spent bleaching clay; shiitake mushroom; Lentinula edodes; vegetable oils; spawn production; mycelial growth; components
{Abstract}: While no effective (both in a technical and economic sense) methods exist so far to handle large quantities of untreated spent bleaching clay (SBC) in China, there are indeed great demands for low-cost alternatives for mushroom substrate. Hence, it is in the interest of both edible-oil-refining industries and mushroom producers to explore how partially substituting traditional shiitake substrate by SBC would influence the mycelial growth in shiitake spawn production. This paper conducted a comparative shiitake spawn production experiment with a conventional shiitake substrate formula comprising of 78% sawdust, 20% wheat bran, 1% magnesium sulfate, and 1% gypsum (percentage by weight, the same thereafter) as the control and substrate formulae with the substitution of SBC for part of sawdust or wheat bran as treatments to observe the mycelial growth. The results indicated that formulae with SBC had lower incidence of microbial contamination and higher mycelial growth speed, and the optimal formula was 15% SBC, 78% coarse sawdust, 5% wheat bran, 1% magnesium sulfate and 1% gypsum. Therefore, the utilization of SBC in shiitatke spawn production not only considerably reduces the costs of shiitake production but also bring about substantial environmental benefits.
{Author Address}: SW Jiaotong Univ, Sch Environm Sci & Engn, Chengdu 610031, Peoples R China; Chengdu Univ, Coll Rural & Urban Construct, Chengdu 610106, Peoples R China; SW Jiaotong Univ, Sch Environm Sci & Engn, Chengdu 610031, Peoples R China; Sichuan Univ, Coll Architecture & Environm, Chengdu 610065, Peoples R China; Chinese Acad Sci, Chengdu Inst Mt Hazards & Environm, Chengdu 610041, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China; Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Dundar, Abdurrahman; Acay, Hilal; Yildiz, Abdunnasir
{Year}: 2009
{Title}: Effect of using different lignocellulosic wastes for cultivation of Pleurotus ostreatus (Jacq.) P. Kumm. on mushroom yield, chemical composition and nutritional value
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 8
{Issue}: 4
{Pages}: 662-666
{ISBN/ISSN}: 1684-5315
{Keywords}: AGRICULTURAL WASTES; EDIBLE MUSHROOMS; QUALITY; SINGER; STRAINS; Pleurotus ostreatus; amino acid; chemical composition; nutritional value; lignocellulosic wastes
{Abstract}: In this study, the mushroom yield, chemical composition and nutritional value of Pleurotus ostreatus (Jacq.) P. Kumm. cultivated in wheat stalk (WS), millet stalk (MS), soybean stalk (SS) and cotton stalk (CS) were determined. Fresh mushroom yield amounts (100 g of substrate, 70% moisture) obtained from WS, CS, MS and SS substrate media were 17.9, 14.3, 22.7 and 31.5 g, respectively. Samples of mushroom cultivated on different culture mediums were analysed for protein, energy, ash, fat, dietary fibre, carbohydrate, moisture, vitamins (thiamin, riboflavin, pyridoxin and niacin) and amino acid contents.
{Author Address}: Dicle Univ, Inst Sci, Dept Biol, TR-21280 Diyarbakir, Turkey; Dicle Univ, Inst Sci, Dept Biol, TR-21280 Diyarbakir, Turkey; Dicle Univ, Fac Sci & Arts, Dept Biol, TR-21280 Diyarbakir, Turkey
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Turkey; Turkey

{Reference Type}: Journal Article
{Author}: Das, S. K.; Masuda, M.; Sakurai, A.; Sakakibara, M.
{Year}: 2009
{Title}: Effects of additives on cordycepin production using a Cordyceps militaris mutant induced by ion beam irradiation
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 8
{Issue}: 13
{Pages}: 3041-3047
{ISBN/ISSN}: 1684-5315
{Keywords}: SUBMERGED CULTIVATION; CULTURE; Ion beam; cordycepin; Cordyceps militaris; mutant; additives
{Abstract}: To obtain a sustainable mutant of the medicinal mushroom Cordyceps militaris with a higher cordycepin production, high-energy ion beam irradiation was applied in the present study. Upon successful irradiation by a proton beam, 30 classes of 8-azaadenine and 28 classes of 8-azaaguanine resistant mutants were obtained of which 7 classes (A63-7, A63-8, A81-2, A81-6, G63-8, G81-3, G82-4) were selected as initially promising mutants using their antibacterial ability as an index of cordycepin production. Among these mutants, G81-3 had the highest cordycepin production of 6.84 g/l using optimized conditions compared to that of the control of 2.45 g/l (2.79 times higher). In addition, to explore the influences of different additives on the cordycepin production using the above mutant in a surface liquid culture, adenosine and glycine were used as additives. In the culture medium under the previously optimized conditions for the said mutant, 2, 4, 6, 8 and 10 g/l adenosine were separately added. These results revealed the highest cordycepin production of 8.57 g/l when using 6 g/l adenosine was 28.10% higher than that of the control (6.69 g/l). This is a highest report of cordycepin production until now. Similarly, the results of other concentrations also superseded the control. The time course of glucose showed that the glucose consumption for the 4 g/l adenosine was the fastest, while that of 10 g/l was the slowest with the longest culture time among all the treatments. For the same purpose, glycine was used with yeast extract in weight percent ratios (yeast extract/glycine) of 40/60, 50/50, 60/40, 70/30, 80/20 and 90/10 under the condition that the total amount of glycine and yeast extract were fixed. Also, the glycine was separately added as 10, 20 and 30 weight percent of yeast extract in the culture medium having the same optimized conditions with a fixed yeast extract concentration. These results showed that the 90/10 ratio had the best cordycepin production of 6.80 g/l that was 12.40% higher versus the control (6.05 g/l). The cordycepin production of the 70/30 and 80/20 weight percent ratios were also higher than that of the control, while the others had a lower cordycepin production compared to that of the control; especially the cordycepin production with the 10, 20 and 30 weight percent yeast extracts inversely decreased in accordance with the used glycine concentration. Regarding the time course, the glucose consumption for the 40/60 weight percent ratio of yeast extract was the fastest, while that of the 30 weight percent was the slowest with the longest culture time among all the treatments. These results suggested that both the ion beam irradiation and additives had active influences on the cordycepin production and that adenosine had a much better influence than that of glycine. It was also evident that a higher concentration of both adenosine and glycine negatively affected the cordycepin production.
{Author Address}: Univ Fukui, Grad Sch Engn, Dept Appl Chem & Biotechnol, Fukui 9108507, Japan; Univ Fukui, Grad Sch Engn, Dept Appl Chem & Biotechnol, Fukui 9108507, Japan; Univ Fukui, Grad Sch Engn, Dept Appl Chem & Biotechnol, Fukui 9108507, Japan; Univ Fukui, Grad Sch Engn, Dept Appl Chem & Biotechnol, Fukui 9108507, Japan
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Japan