{Reference Type}: Journal Article
{Author}: Kajihara, Ikko; Jinnin, Masatoshi; Yamane, Keitaro; Makino, Takamitsu; Honda, Noritoshi; Igata, Toshikatsu; Masuguchi, Shinichi; Fukushima, Satoshi; Okamoto, Yoshinobu; Hasegawa, Minoru; Fujimoto, Manabu; Ihn, Hironobu
{Year}: 2012
{Title}: Increased Accumulation of Extracellular Thrombospondin-2 Due to Low Degradation Activity Stimulates Type I Collagen Expression in Scleroderma Fibroblasts
{Tag}: 0
{Star}: 0
{Journal}: AMERICAN JOURNAL OF PATHOLOGY
{Volume}: 180
{Issue}: 2
{Pages}: 703-714
{ISBN/ISSN}: 0002-9440
{Keywords}: GROWTH-FACTOR-BETA; SYSTEMIC-SCLEROSIS FIBROBLASTS; HUMAN DERMAL FIBROBLASTS; CELL-MATRIX INTERACTIONS; SKIN FIBROBLASTS; IMMUNE-SYSTEM; III COLLAGEN; ACTIVATION; FIBROSIS; PATHOGENESIS
{Abstract}: The aim of the present study was to determine the expression and role of thrombospondin-2 (TSP-2) in systemic sclerosis (SSc). Both TSP-2 mRNA levels and protein synthesis in cell lysates were significantly lower in cultured SSc fibroblasts than in normal fibroblasts; however, the TSP-2 protein that accumulated in the conditioned medium of SSc fibroblasts was up-regulated, compared with that of normal fibroblasts, because of an increase in the half-life of the protein. In vivo serum TSP-2 levels were higher in SSc patients than in healthy control subjects, and SSc patients with elevated serum TSP-2 levels tended to have pitting scars and/or ulcers. TSP-2 knockdown resulted in the down-regulation of type I collagen expression and the up-regulation of miR-7, one of the miRNAs with an inhibitory effect on collagen expression. Expression levels of miR-7 were also up-regulated in SSc dermal fibroblasts both in vivo and in vitro. Taken together, these findings suggest that the increased extracellular TSP-2 deposition in SSc fibroblasts may contribute to tissue fibrosis by inducing collagen expression. Down-regulation of intracellular TSP-2 synthesis and the subsequent miR-7 up-regulation in SSc fibroblasts may be due to a negative feedback mechanism that prevents increased extracellular TSP-2 deposition and/or tissue fibrosis. Thus, TSP-2 may play an important role in the maintenance of fibrosis and angiopathy in patients with SSc.(Am J Patbol 2012, 180: 703-714; DOI: 10.1016/j.ajpath.2011.10.030)
{Author Address}: Kumamoto Univ, Dept Dermatol & Plast Surg, Fac Life Sci, Kumamoto, Japan; Kumamoto Univ, Dept Dermatol & Plast Surg, Fac Life Sci, Kumamoto, Japan; Kumamoto Univ, Dept Dermatol & Plast Surg, Fac Life Sci, Kumamoto, Japan; Kumamoto Univ, Dept Dermatol & Plast Surg, Fac Life Sci, Kumamoto, Japan; Kumamoto Univ, Dept Dermatol & Plast Surg, Fac Life Sci, Kumamoto, Japan; Kumamoto Univ, Dept Dermatol & Plast Surg, Fac Life Sci, Kumamoto, Japan; Kumamoto Univ, Dept Dermatol & Plast Surg, Fac Life Sci, Kumamoto, Japan; Kumamoto Univ, Dept Dermatol & Plast Surg, Fac Life Sci, Kumamoto, Japan; Kanazawa Univ, Dept Dermatol, Grad Sch Med Sci, Kanazawa, Ishikawa, Japan; Kanazawa Univ, Dept Dermatol, Grad Sch Med Sci, Kanazawa, Ishikawa, Japan; Kanazawa Univ, Dept Dermatol, Grad Sch Med Sci, Kanazawa, Ishikawa, Japan; Kumamoto Univ, Dept Dermatol & Plast Surg, Fac Life Sci, Kumamoto, Japan
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Japan; Japan

{Reference Type}: Journal Article
{Author}: Shimada, Tsuyoshi; Hiramatsu, Nobuhiko; Kasai, Ayumi; Mukai, Mai; Okamura, Maro; Yao, Jian; Huang, Tao; Tamai, Minori; Takahashi, Shuhei; Nakamura, Tomoyuki; Kitamura, Masanori
{Year}: 2008
{Title}: Suppression of adipocyte differentiation by Cordyceps militaris through activation of the aryl hydrocarbon receptor
{Tag}: 0
{Star}: 0
{Journal}: AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
{Volume}: 295
{Issue}: 4
{Pages}: E859-E867
{ISBN/ISSN}: 0193-1849
{Keywords}: MONOCYTE CHEMOATTRACTANT PROTEIN-1; ENDOPLASMIC-RETICULUM STRESS; NF-KAPPA-B; ADIPOSE DIFFERENTIATION; 3T3-L1 CELLS; 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN TCDD; ARYLHYDROCARBON RECEPTOR; DEPENDENT ACTIVATION; INSULIN-RESISTANCE; PHELLINUS-LINTEUS; mycelium; adipogenesis; 3T3-L1 cells
{Abstract}: Mycelial extracts have a wide range of biological activities that modulate functions of mammalian cells. In this report, we sought to identify antiadipogenic mycelia with the use of 3T3-L1 cells and found that the extract of Cordyceps militaris exclusively suppressed differentiation of 3T3-L1 preadipocytes into mature adipocytes without affecting cell viability. This inhibitory effect was dose dependent, reversible, and associated with 1) a decrease in lipid accumulation, 2) blunted induction of adipocyte markers including adiponectin, peroxisome proliferator-activated receptor-gamma, and CCAAT/enhancer binding protein-alpha, and 3) sustained expression of a preadipocyte marker, monocyte chemoattractant protein-1. C. militaris also significantly decreased accumulation of lipid and hypertrophy in mature adipocytes and preserved their response to insulin (phosphorylation of Akt) during prolonged culture. Subsequent experiments revealed that C. militaris has the potential to activate the aryl hydrocarbon receptor (AhR). In 3T3-L1 cells, treatment with AhR agonists including benzo[a] pyrene and 3-methylcholanthrene reproduced the antiadipogenic effect of C. militaris. Furthermore, dominant-negative inhibition of AhR abrogated the suppressive effect of C. militaris on adipocyte differentiation. These results suggest that C. militaris has the potential to interfere with adipocyte differentiation through activation of AhR.
{Author Address}: Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Mol Signaling, Yamanashi 4093898, Japan; Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Mol Signaling, Yamanashi 4093898, Japan; Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Mol Signaling, Yamanashi 4093898, Japan; Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Mol Signaling, Yamanashi 4093898, Japan; Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Mol Signaling, Yamanashi 4093898, Japan; Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Mol Signaling, Yamanashi 4093898, Japan; Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Mol Signaling, Yamanashi 4093898, Japan; Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Mol Signaling, Yamanashi 4093898, Japan; Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Mol Signaling, Yamanashi 4093898, Japan; IBI Corp, Appl Fungi Inst, Yamanashi, Japan; Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Mol Signaling, Yamanashi 4093898, Japan
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Japan; Japan

{Reference Type}: Journal Article
{Author}: Kim, S. W.; Kim, M. G.; Jung, H. A.; Lee, K. H.; Lee, H. S.; Ro, H. S.
{Year}: 2008
{Title}: An application of protein microarray in the screening of monoclonal antibodies against the oyster mushroom spherical virus
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18191463&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Anal Biochem
{Volume}: 374
{Issue}: 2
{Pages}: 313-7
{DOI}: 10.1016/j.ab.2007.12.010
{Date Displayed}: 2008 Mar 15
{Date}: 2008-03-15
{Type of Work}: Journal Article; Research Support, Non-U.S. Gov't
{Accession Number}: 18191463
{Keywords}: Animals; Antibodies, Monoclonal/*analysis/*immunology; Antibody Specificity/*immunology; Female; Hybridomas/immunology/metabolism; Immunoblotting; Immunoglobulin G/immunology; Mice; Mice, Inbred BALB C; Pleurotus/*virology; *Protein Array Analysis; Protein Structure, Tertiary; Viral Proteins/chemistry/genetics/immunology; Viruses/genetics/*immunology
{Abstract}: The oyster mushroom spherical virus (OMSV) is a causative agent of dieback disease in the oyster mushroom, Pleurotus ostreatus. Outbreaks of this virus occasionally result in serious disease that is associated with hefty economic losses. Thus, the detection and removal of OMSV-infected spawn is considered to be a crucial step for the stable production of P. ostreatus. For the detection of OMSV, we attempted to generate monoclonal antibodies (mAbs) against an RNA polymerase domain (RPD) of an OMSV protein. In an effort to simplify the laborious multistep mAb screening process, we developed a protein microarray on a slide glass that is chemically modified with the RPD protein. The culture supernatants of 87 hybridoma cells, which were prepared from the fusion of RPD-immunized mouse spleen cells with myeloma cells, were spotted onto the RPD-coated microarray. The binding of mAb to RPD was detected via Alexa 488 dye-labeled anti-mouse immunoglobulin G (IgG) as a secondary antibody. Of 87 samples, 13 evidenced a significant level of fluorescence signal intensity. Subsequent immunoblot analysis revealed that the specificity of each mAb against   RPD coincided with the corresponding fluorescence signal intensity, thereby indicating the effectiveness of the protein microarray in mAb screening.
{Author Address}: Deparment of Microbiology and Research Institute of Life Science, Gyeongsang National University, Chinju 660-701, Korea.
{Language}: eng

{Reference Type}: Journal Article
{Author}: Liu, P.; Li, Y. Y.; Li, H. M.; Wan, D. J.; Tang, Y. J.
{Year}: 2011
{Title}: Determination of the nucleosides and nucleobases in Tuber samples by dispersive solid-phase extraction combined with liquid chromatography-mass spectrometry
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=21277418&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Anal Chim Acta
{Volume}: 687
{Issue}: 2
{Pages}: 159-67
{DOI}: 10.1016/j.aca.2010.12.025
{Date Displayed}: 2011 Feb 21
{Date}: 2011-02-21
{Type of Work}: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies
{Original Publication}: Copyright (c) 2010 Elsevier B.V. All rights reserved.
{Accession Number}: 21277418
{Keywords}: Adenine/analysis; Adenosine/analysis; Agaricales/*chemistry/*genetics; Chromatography, High Pressure Liquid/methods; Chromatography, Liquid/methods; Fermentation; Guanine/analysis; Guanosine/analysis; Hypoxanthine/analysis; Inosine/analysis; Mass Spectrometry/methods; Nucleosides/*analysis; Plant Extracts/*chemistry; Plant Tubers/*chemistry; Solid Phase Extraction/*methods; Tandem Mass Spectrometry/*methods; Uridine/analysis
{Abstract}: A simple, fast and inexpensive method based on dispersive solid phase extraction   (DSPE) combined with LC-MS was developed for simultaneous determination of 7 nucleosides and nucleobases (i.e., adenine, hypoxanthine, uridine, adenosine, guanine, guanosine, and inosine) in Tuber fruiting-bodies and fermentation mycelia. The DSPE procedure was firstly introduced to remove the protein interference from sample solutions, and D3520 macroporous resin was chosen as the DSPE sorbent because of its high removal capability on protein interferences, but low adsorption rate on analytes. Besides, key parameters on DSPE procedure (i.e., macroporous resin type, macroporous resin amount, methanol concentration, and vortex time) were optimized, and the protein removal efficacy could achieve about 95% after the process optimization. Though the method validation test, the DSPE-LC-MS method was confirmed to be precise, accurate and sensitive, and the column blinding problem was solved successfully. By using this established method, the total amount of nucleosides and nucleobases in the fermentation mycelia was determined to range from 4881.5 to 12,592.9mugg(-)(1), which was about 2-25 times higher than the fruiting-bodies (from 498.1 to 2274.1mugg(-)(1)). The formulation of nucleosides and nucleobases in the fermentation mycelia maintained relatively constant, while the formulation in Tuber fruiting-bodies varied significantly with their species. Hierarchical cluster analysis (HCA) showed the formulation similarity of nucleosides and nucleobases between Tuber fermentation mycelia and the fruiting-bodies of Tuber indicum and Tuber himalayense. From the viewpoint of nucleosides and nucleobases, this work confirms the potentiality of Tuber fermentation mycelia as the alternative resource for its fruiting-bodies.
{Author Address}: Hubei University of Technology, Wuhan, China.
{Language}: eng