{Reference Type}: Journal Article
{Author}: Liu, Yanbin; Koh, Chong Mei John; Sun, Longhua; Hlaing, Mya Myintzu; Du, Minge; Peng, Ni; Ji, Lianghui
{Year}: 2013
{Title}: Characterization of glyceraldehyde-3-phosphate dehydrogenase gene RtGPD1 and development of genetic transformation method by dominant selection in oleaginous yeast Rhodosporidium toruloides
{Tag}: 0
{Star}: 0
{Journal}: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
{Volume}: 97
{Issue}: 2
{Pages}: 719-729
{ISBN/ISSN}: 0175-7598
{Keywords}: USTILAGO-MAYDIS; PHANEROCHAETE-CHRYSOSPORIUM; SCHIZOPHYLLUM-COMMUNE; RECOMBINANT PROTEIN; AGARICUS-BISPORUS; PROMOTER; EXPRESSION; AGROBACTERIUM; ACTIVATION; CLONING; Glyceraldehyde-3-phosphate dehydrogenase; Oleaginous yeast; Rhodosporidium toruloides; Agrobacterium tumefaciens-mediated transformation; Microbial oil
{Abstract}: The oleaginous yeast Rhodosporidium toruloides, which belongs to the Pucciniomycotina subphylum in the Basidiomycota, has attracted strong interest in the biofuel community recently due to its ability to accumulate more than 60% of dry biomass as lipid under high-density fermentation. A 3,543-nucleotide (nt) DNA fragment of the glyceraldehyde-3-phosphate dehydrogenase gene (GPD1) was isolated from R. toruloides ATCC 10657 and characterized in details. The 1,038-nt mRNA derived from seven exons encodes an open reading frame (ORF) of 345 amino acids that shows high identity (80%) to the Ustilago maydis homolog. Notably, the ORF is composed of codons strongly biased towards cytosine at the Wobble position. GPD1 is transcriptionally regulated by temperature shock, osmotic stress, and carbon source. Nested deletion analysis of the GPD1 promoter by GFP reporter assay revealed that two regions, -975 to -1,270 and -1,270 to -1,429, upstream from the translational start site of GPD1 were important for responses to various stress stimuli. Interestingly, a 176-bp short fragment maintained 42.2% promoter activity of the 795-bp version in U. maydis whereas it was reduced to 17.4% in R. toruloides. The GPD1 promoter drove strong expression of a codon-optimized enhanced green fluorescent protein gene (RtGFP) and a codon-optimized hygromycin phosphotransferase gene (hpt-3), which was critical for Agrobacterium tumefaciens-mediated transformation in R. toruloides.
{Author Address}: Natl Univ Singapore, Temasek Life Sci Lab, Biomat & Biocatalysts Grp, Singapore 117604, Singapore; Natl Univ Singapore, Temasek Life Sci Lab, Biomat & Biocatalysts Grp, Singapore 117604, Singapore; Natl Univ Singapore, Temasek Life Sci Lab, Biomat & Biocatalysts Grp, Singapore 117604, Singapore; Natl Univ Singapore, Temasek Life Sci Lab, Biomat & Biocatalysts Grp, Singapore 117604, Singapore; Natl Univ Singapore, Temasek Life Sci Lab, Biomat & Biocatalysts Grp, Singapore 117604, Singapore; Natl Univ Singapore, Temasek Life Sci Lab, Biomat & Biocatalysts Grp, Singapore 117604, Singapore; Natl Univ Singapore, Temasek Life Sci Lab, Biomat & Biocatalysts Grp, Singapore 117604, Singapore
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Singapore

{Reference Type}: Journal Article
{Author}: Ando, Yuki; Nakazawa, Takehito; Oka, Kunihiko; Nakahori, Kiyoshi; Kamada, Takashi
{Year}: 2013
{Title}: Cc.snf5, a gene encoding a putative component of the SWI/SNF chromatin remodeling complex, is essential for sexual development in the agaricomycete Coprinopsis cinerea
{Tag}: 0
{Star}: 0
{Journal}: FUNGAL GENETICS AND BIOLOGY
{Volume}: 50
{Pages}: 82-89
{ISBN/ISSN}: 1087-1845
{Keywords}: BASIDIOMYCETE COPRINUS-CINEREUS; IN-VIVO; MULTISUBUNIT COMPLEX; MORPHOGENESIS; RECRUITMENT; MUTAGENESIS; MUTATIONS; PRODUCTS; MUTANTS; DOMAINS; Coprinus cinereus; Dikaryon; Fruiting; Gene targeting; REMI
{Abstract}: We characterized a Coprinopsis cinerea mutant strain, Spe20, defective in fruiting initiation, which was isolated after restriction enzyme-mediated integration (REMI) mutagenesis of a homokaryotic fruiting strain, 326. A plasmid rescue followed by complementation experiments, RACE, and cDNA analyses revealed that the gene, a mutation of which is responsible for the phenotype, is predicted to encode a protein that exhibits a high similarity to yeast Snf5p, a key component of the chromatin remodeling complex SWI/SNF, and named Cc.snf5. Cc.Snf5 is, however, different from Snf5p in that the former has, in addition to an Snf5 domain comprising N-terminal repeat1 (rp1) and C-terminal repeat2 (rp2) subdomains in a middle region, a GATA Zn-finger domain in a C-terminal region. In strain Spe20, plasmid pPHT1 used for REMI is inserted in the ORF encoding rp2. This raised the possibility that in strain Spe20, the disrupted Cc.Snf5 is functionally active albeit incompletely because it retains rp1. Thus, we disrupted the whole SNF5 domain and its downstream peptide and found that the disruption results in inhibition of not only fruiting initiation but also dikaryon development, a prerequisite for fruiting. We also found that specific disruption of the Zn-finger domain results in inhibition of fruiting initiation. These results indicate that Cc.Snf5 plays an essential role in sexual development of C cinerea. (C) 2012 Elsevier Inc. All rights reserved.
{Author Address}: <AuCollectiveName>Ando, Yuki</AuCollectiveName>
</fullauthorname>
<author>Nakazawa, T</author>
<fullauthorname>
<AuRole>Author</AuRole>
<AuLastName>Nakazawa</AuLastName>
<AuFirstName>Takehito</AuFirstName>
<address number=""/>
<AuCollectiveName>Nakazawa, Takehito</AuCollectiveName>
</fullauthorname>
<author>Oka, K</author>
<fullauthorname>
<AuRole>Author</AuRole>
<AuLastName>Oka</AuLastName>
<AuFirstName>Kunihiko</AuFirstName>
<address number=""/>
<AuCollectiveName>Oka, Kunihiko</AuCollectiveName>
</fullauthorname>
<author>Nakahori, K</author>
<fullauthorname>
<AuRole>Author</AuRole>
<AuLastName>Nakahori</AuLastName>
<AuFirstName>Kiyoshi</AuFirstName>
<address number=""/>
<AuCollectiveName>Nakahori, Kiyoshi</AuCollectiveName>
</fullauthorname>
<author>Kamada, T</author>
<fullauthorname>
<AuRole>Author</AuRole>
<AuLastName>Kamada</AuLastName>
<AuFirstName>Takashi</AuFirstName>
<address number="1">Okayama Univ, Fac Sci, Dept Biol, Grad Sch Nat Sci &amp; Technol, Okayama 7008530, Japan
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Japan

{Reference Type}: Journal Article
{Author}: Ge, Zai-Wei; Smith, Matthew E.
{Year}: 2013
{Title}: Phylogenetic analysis of rDNA sequences indicates that the sequestrate Amogaster viridiglebus is derived from within the agaricoid genus Lepiota (Agaricaceae)
{Tag}: 0
{Star}: 0
{Journal}: MYCOLOGICAL PROGRESS
{Volume}: 12
{Issue}: 1
{Pages}: 151-155
{ISBN/ISSN}: 1617-416X
{Keywords}: COMBINATIONS; MACROLEPIOTA; BOOTSTRAP; Agaricales; Fungal systematics; Phylogeny; Lepiota; Sequestrate fungi
{Abstract}: The rare sequestrate fungus Amogaster viridiglebus is known from only one collection in California where it was discovered among Populus roots. Based on sporocarp coloration and spore morphology, this sequestrate taxon was putatively considered to be an ectomycorrhizal member of the Boletales. However, no molecular data were previously available to definitively determine the closest relatives of this fungus. Here we revisit the morphology of Amogaster viridiglebus and present a phylogenetic analysis based on ITS and 28S ribosomal DNA. Our phylogeny indicates that Amogaster viridiglebus is nested in the genus Lepiota, suggesting that this rare species has a saprobic trophic mode and does not form ectomycorrhizae with plants. A new combination, L. viridigleba, is made based on these phylogenetic results.
{Author Address}: Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA; Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China; Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: USA; Peoples R China