{Reference Type}: Journal Article
{Author}: Joseph, Soniamol; Sabulal, Baby; George, Varughese; Antony, Kuttikkadan Rony; Janardhanan, Kainoor Krishnankutty
{Year}: 2011
{Title}: Antitumor and anti-inflammatory activities of polysaccharides isolated from Ganoderma lucidum
{Tag}: 0
{Star}: 0
{Journal}: ACTA PHARMACEUTICA
{Volume}: 61
{Issue}: 3
{Pages}: 335-342
{ISBN/ISSN}: 1330-0075
{Keywords}: LENTINUS-EDODES; CANCER CELLS; Ganoderma lucidum (Polyporaceae); polysaccharides; anti-inflammatory activity; antitumor activity
{Abstract}: In this study, polysaccharides were isolated from Ganoderma lucidum (Polyporaceae) and their antitumor and anti-inflammatory activities were investigated using in vivo models. Potential antitumor activity was shown by G. lucidum polysaccharides (GLP) against solid tumor induced by Ehrlich's ascites carcinoma cells. GLP at 100 mg kg(-1) body mass showed 80.8 and 77.6% reduction in tumour volume and tumour mass, respectively, when administered 24 h after tumour implantation. Again, GLP at the same dose but when administered prior to tumour inoculation, showed 79.5 and 81.2% inhibition of tumour volume and tumour mass, respectively. GLP showed significant dose-dependent activity in carrageenean-induced (acute) and formalin-induced (chronic) inflammation assays. At 100 mg kg(-1), GLP exhibited 57.6 and 58.2% inhibition in carrageenean-induced and formalin-induced assays, respectively.
{Author Address}: Trop Bot Garden & Res Inst, Phytochem & Phytopharmacol Div, Thiruvananthapuram 695562, Kerala, India; Trop Bot Garden & Res Inst, Phytochem & Phytopharmacol Div, Thiruvananthapuram 695562, Kerala, India; Amity Inst Herbal & Biotech Prod Dev, Thiruvananthapuram 695005, Kerala, India; Trop Bot Garden & Res Inst, Phytochem & Phytopharmacol Div, Thiruvananthapuram 695562, Kerala, India; Amala Canc Res Ctr, Amala Nagar 680555, Thrissur, India; Amala Canc Res Ctr, Amala Nagar 680555, Thrissur, India
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: India; India; India

{Reference Type}: Journal Article
{Author}: Sulkowska-Ziaja, Katarzyna; Karczewska, Elzbieta; Wojtas, Izabela; Budak, Alicja; Muszynska, Bozena; Ekiert, Halina
{Year}: 2011
{Title}: ISOLATION AND BIOLOGICAL ACTIVITIES OF POLYSACCHARIDE FRACTIONS FROM MYCELIUM OF SARCODON IMBRICATUS L. P. KARST. (BASIDIOMYCOTA) CULTURED IN VITRO
{Tag}: 0
{Star}: 0
{Journal}: ACTA POLONIAE PHARMACEUTICA
{Volume}: 68
{Issue}: 1
{Pages}: 143-145
{ISBN/ISSN}: 0001-6837
{Keywords}: ANTITUMOR-ACTIVITY; ANTIOXIDANT; ITO; mycelial culture; Basidiomycota; polysaccharides; antimicrobial activity
{Author Address}: Jagiellonian Univ, Coll Med, Chair Pharmaceut Bot, PL-30688 Krakow, Poland; Jagiellonian Univ, Coll Med, Dept Pharmaceut Bot, PL-30688 Krakow, Poland; Jagiellonian Univ, Coll Med, Dept Pharmaceut, PL-30688 Krakow, Poland; Jagiellonian Univ, Coll Med, Dept Pharmaceut, PL-30688 Krakow, Poland; Jagiellonian Univ, Coll Med, Dept Pharmaceut, PL-30688 Krakow, Poland; Jagiellonian Univ, Coll Med, Chair Pharmaceut Bot, PL-30688 Krakow, Poland; Jagiellonian Univ, Coll Med, Dept Pharmaceut Bot, PL-30688 Krakow, Poland; Jagiellonian Univ, Coll Med, Chair Pharmaceut Bot, PL-30688 Krakow, Poland; Jagiellonian Univ, Coll Med, Dept Pharmaceut Bot, PL-30688 Krakow, Poland
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Poland; Poland; Poland

{Reference Type}: Journal Article
{Author}: Wang, Yi-feng; Lian, Xin-xin; Wu, Zhong-kui; Xiong, Yan-fei
{Year}: 2012
{Title}: RESEARCH ON THE SURFACE PROPERTIES OF POLY(LACTIC ACID) MODIFIED BY CARBOXYMETHYLATED LENTINAN
{Tag}: 0
{Star}: 0
{Journal}: ACTA POLYMERICA SINICA
{Issue}: 10
{Pages}: 1164-1169
{ISBN/ISSN}: 1000-3304
{Keywords}: POLYSACCHARIDES; EDODES; Poly(lactic acid); Carboxymethylated lentinan; Surface modification; Chemical grafting; Layer-by-layer assembly
{Abstract}: Lentinan, a mushroom polysaccharide, isolated from Lentinus edodes (Shiitake mushroom) was carboxymethylated in NaOH and isopropanol solution to obtain a water-soluble derivative. Then, carboxymethylated lentinan was covalently immobilized onto the surface of aminated poly(lactic acid) (PLA) substrates via chemical grafting method. In addition, carboxymethylated lentinan and chitosan were alternatively deposited on the aminated PLA substrates via layer-by-layer (LbL) assembly method, in order to construct a polysaccharide-based multilayer on the substrates. The carboxymethylated lentinan was proved by infrared spectroscopy. The degree of substitution and molecular weight (M-w) of carboxymethylated lentinan were determined by UV spectrophotometry and gel permeation chromatography (GPC), respectively. The modified and unmodified PLA surfaces were investigated by scanning electron microscope (SEM) and water contact angle measurements. The hemolysis test, thrombus test, and the antibacterial activity against Escherichia coli of modified and unmodified PLA were tested in vitro, respectively. Then, the surface properties of PLA modified by different modification methods were evaluated and compared. The results showed that the hydrophilicity, blood compatibility, and antibacterial activity against Escherichia coli of PLA modified by LbL assembly and chemical grafting were both improved. In addition, PLA modified by carboxymethylated lentinan and chitosan through LbL assembly method showed better hydrophilicity,, blood compatibility, and antibacterial activity against Escherichia coli, in comparison with that modified by chemical grafting method.
{Author Address}: Wuhan Univ Technol, Sch Mat Sci & Engn, Dept Polymer Mat & Engn, Wuhan 430070, Peoples R China; Wuhan Univ Technol, Sch Mat Sci & Engn, Dept Polymer Mat & Engn, Wuhan 430070, Peoples R China; Wuhan Univ Technol, Sch Mat Sci & Engn, Dept Polymer Mat & Engn, Wuhan 430070, Peoples R China; Wuhan Univ Technol, Sch Sci, Dept Biol Sci & Technol, Wuhan 430070, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: Chinese
{Country}: Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Sheng, Li; Shuqin, Xu; Lina, Zhang
{Year}: 2010
{Title}: ADVANCES IN CONFORMATIONS AND CHARACTERIZATIONS OF FUNGI POLYSACCHARIDES
{Tag}: 0
{Star}: 0
{Journal}: ACTA POLYMERICA SINICA
{Issue}: 12
{Pages}: 1359-1375
{ISBN/ISSN}: 1000-3304
{Keywords}: ATOMIC-FORCE MICROSCOPY; SCHIZOPHYLLUM-COMMUNE POLYSACCHARIDE; AQUEOUS SODIUM-CHLORIDE; TRIPLE-HELICAL POLYSACCHARIDE; PLEUROTUS-TUBER-REGIUM; PORIA-COCOS MYCELIA; MOUSE PERITONEAL-MACROPHAGES; COIL-GLOBULE TRANSITION; DOUBLE-STRANDED HELIX; BETA-GLUCAN RECEPTOR; Fungi polysaccharides; Chain conformation; Molecular weight; Characterization methods; Bioactivities
{Abstract}: This paper summarizes the current research results on the chain conformations of the fungi polysaccharides in solution and characterization methods. The molecular weight, particle size, and chain conformation of various polysaccharides exacted from mushrooms including Lentinus edodes, Poria cocos, Ganoderma lucidum, Auricularia auricula-judae, Xanthomonas, Schizophyllan, etc. in solution are introduced, and the relationships between the bioactivity and the chain conformation are outlined. It is concluded that extended chain, charged groups and moderate molecular weight of the polysaccharides are beneficial to enhance the antitumor activities, as results of the increasing the interaction between polysaccharide molecules and immune system. It is indicated the importance of understanding the relationship between polysaccharide chain conformation and biological activity. Polysaccharides mainly exist as random coil, double helix, triple helix, the worm-like and rod-like chain conformations, as well as aggregates in solution, depending on the type of monosaccharide and glycosidic link, the branch chain structure and intermolecular interactions. The major characterization methods include light scattering, viscosity, microscopy (TEM, SEM and atomic force microscope), microcalorimetry and small angle X-ray scattering. In addition, the polysaccharides solution theory and the calculation expression of chain conformation parameters are represented and discussed.
{Author Address}: Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China; Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China; Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: Chinese
{Country}: Peoples R China