{Reference Type}: Journal Article
{Author}: Yan, Jing-Kun; Wang, Wen-Qiang; Ma, Hai-Le; Wu, Jian-Yong
{Year}: 2013
{Title}: Sulfation and Enhanced Antioxidant Capacity of an Exopolysaccharide Produced by the Medicinal Fungus Cordyceps sinensis
{Tag}: 0
{Star}: 0
{Journal}: MOLECULES
{Volume}: 18
{Issue}: 1
{Pages}: 167-177
{ISBN/ISSN}: 1420-3049
{Keywords}: IN-VITRO; CHEMICAL-COMPOSITION; ANTITUMOR ACTIVITIES; MYCELIAL CULTURE; POLYSACCHARIDES; ANTICOAGULANT; CARRAGEENANS; RADICALS; RED; Cordyceps sinensis; exopolysaccharide; sulfation; molecular weight; antioxidant activity
{Abstract}: EPS-1 was an exopolysaccharide produced by the medicinal fungus Cordyceps sinensis (Cs-HK1). In the present study, EPS-1 was sulfated with chlorosulfonic acid (CSA)-pyridine (Pyr) at different volume ratios, yielding four sulfated derivatives, SEPS-1A, B, C and D, with different degrees of substitution (DS: 0.25-1.38) and molecular weights (17.1-4.1 kDa). The sulfation of EPS-1 occurred most frequently at the C-6 hydroxyl groups due to their higher reactivity. In aqueous solution, the native EPS-1 formed random coils or aggregated networks, but the sulfated derivatives formed single helices. The antioxidant activities of the sulfated EPS-1 derivatives for scavenging hydroxyl radicals (center dot OH) and 2,2-azinobis-3-ehtylbenzothiazolin-6-sulfonic acid radicals (ABTS center dot+) were significantly increased with increasing DS and decreasing molecular weight (MW). Sulfation has thus been shown to be an effective and favorable strategy for improving the physico-chemical properties and bioactivities of fungal polysaccharides.
{Author Address}: Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China; Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China; Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China; Phys Proc Agr Prod Key Lab Jiangsu Prov, Zhenjiang 212013, Jiangsu, Peoples R China; Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Hyun, Sun-Hee; Lee, Seok-Young; Park, Shin Jung; Kim, Da Yeon; Chun, Young-Jin; Sung, Gi-Ho; Kim, Seong Hwan; Choi, Hyung-Kyoon
{Year}: 2013
{Title}: Alteration of Media Composition and Light Conditions Change Morphology, Metabolic Profile, and Beauvericin Biosynthesis in Cordyceps bassiana Mycelium
{Tag}: 0
{Star}: 0
{Journal}: JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
{Volume}: 23
{Issue}: 1
{Pages}: 47-55
{ISBN/ISSN}: 1017-7825
{Keywords}: ENTOMOPATHOGENIC FUNGI; FRUITING BODY; LC-MS/MS; POLYSACCHARIDE; IDENTIFICATION; METARHIZIUM; TELEOMORPH; DISCOVERY; ENNIATINS; SINENSIS; Cordyceps bassiana; mycelium; cultivation condition; metabolic characterization; mass spectrometry
{Abstract}: Metabolic alterations of Cordyceps bassiana mycelium were investigated under the following culture medium and light conditions: dextrose agar supplemented with 0.5% yeast extract (SDAY) medium with light (SL), SDAY medium without light (SD), nut medium without light (ND), and iron-supplemented SDAY medium without light (FD). The levels of asparagine, aspartic acid, glutamic acid, glutamine, histidine, lysine, ornithine, and proline were significantly higher under SD and SL conditions. The levels of most of the alcohols, saturated fatty acids, unsaturated fatty acids, fatty acid esters, sterols, and terpenes were higher under the ND condition than in the other conditions, but beauvericin was not detectable under the ND condition. The FD condition was favorable for the enhanced production of aminomalonic acid, malic acid, mannonic acid, and erythritol. Thus, the metabolic characteristics of C. bassiana can be manipulated by varying the cultivation conditions, rendering this fungus potentially favorable as a nutraceutical and medicinal resource.
{Author Address}: Chung Ang Univ, Coll Pharm, Seoul 156756, South Korea; Chung Ang Univ, Coll Pharm, Seoul 156756, South Korea; Chung Ang Univ, Coll Pharm, Seoul 156756, South Korea; Chung Ang Univ, Coll Pharm, Seoul 156756, South Korea; Chung Ang Univ, Coll Pharm, Seoul 156756, South Korea; Natl Inst Hort & Herbal Sci, RDA, Mushroom Res Div, Dept Herbal Crop Res, Suwon 441707, South Korea; Dankook Univ, Dept Microbiol, Cheonan 330714, South Korea; Chung Ang Univ, Coll Pharm, Seoul 156756, South Korea
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: South Korea; South Korea; South Korea

{Reference Type}: Journal Article
{Author}: Dong, Jing Z.; Ding, J.; Yu, Pei Z.; Lei, Can; Zheng, Xiao J.; Wang, Y.
{Year}: 2013
{Title}: Composition and distribution of the main active components in selenium-enriched fruit bodies of Cordyceps militaris link
{Tag}: 0
{Star}: 0
{Journal}: FOOD CHEMISTRY
{Volume}: 137
{Issue}: 1-4
{Pages}: 164-167
{ISBN/ISSN}: 0308-8146
{Keywords}: LIQUID CULTURE; ANTIOXIDANT; PLANTS; POLYSACCHARIDE; MECHANISMS; EXTRACTION; GROWTH; CANCER; Cordyceps militaris; Selenocompounds; Distribution; Active components; Minerals
{Abstract}: Selenium-enriched Cordyceps militaris fruit bodies are industrially cultivated as functional food or medicinal food in China and southeast Asia. However, composition of selenium compounds and distribution of the main bioactive components are still unknown. In the selenium-enriched fruit bodies, the main soluble selenium compounds of low molecular weight were identified as SeMet (selenomethionine), and the main selenium compounds bound in proteins were identified as SeMet and SeCys (methylselenocysteine). Trace minerals as Se (selenium), Zn (zinc), Fe (iron) and the main active components as adenosine, cordycepin and carotenoids were mostly distributed in the terminal of fruit bodies, while P (phosphorus) and K (potassium) were evenly distributed in the fruit bodies. The results indicated that terminal of the fruit bodies should be the better materials for production of advanced functional food. So cultivation of relatively short and thick fruit bodies with bigger terminals deserves further research. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.
{Author Address}: Hubei Univ Nationalities, Key Lab Biol Resources Protect & Utilizat Hubei P, Sch Biol Sci & Technol, Enshi 445000, Peoples R China; Hubei Univ Nationalities, Key Lab Biol Resources Protect & Utilizat Hubei P, Sch Biol Sci & Technol, Enshi 445000, Peoples R China; Hubei Univ Nationalities, Key Lab Biol Resources Protect & Utilizat Hubei P, Sch Biol Sci & Technol, Enshi 445000, Peoples R China; Hubei Univ Nationalities, Key Lab Biol Resources Protect & Utilizat Hubei P, Sch Biol Sci & Technol, Enshi 445000, Peoples R China; Hubei Univ Nationalities, Key Lab Biol Resources Protect & Utilizat Hubei P, Sch Biol Sci & Technol, Enshi 445000, Peoples R China; Chinese Acad Sci, Key Lab Pant Germplasm Enhancement & Special Agr, Wuhan Bot Garden, Wuhan 430074, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Carneiro, Andreia A. J.; Ferreira, Isabel C. F. R.; Duenas, Montserrat; Barros, Lillian; Da Silva, Roberto; Gomes, Eleni; Santos-Buelga, Celestino
{Year}: 2013
{Title}: Chemical composition and antioxidant activity of dried powder formulations of Agaricus blazei and Lentinus edodes.
{Tag}: 6
{Star}: 0
{Journal}: Food chemistry
{Volume}: 138
{Issue}: 4
{Number}: MEDLINE:23497872
{Pages}: 2168-73
{Cited Count}: 0
{Date Displayed}: 2013
2013-Jun-15
{ISBN/ISSN}: 0308-8146
{Abstract}: Several mushroom species have been pointed out as sources of antioxidant compounds, in addition to their important nutritional value. Agaricus blazei and Lentinus edodes are among the most studied species all over the world, but those studies focused on their fruiting bodies instead of other presentations, such as powdered preparations, used as supplements. In the present work the chemical composition (nutrients and bioactive compounds) and antioxidant activity (free radical scavenging activity, reducing power and lipid peroxidation inhibition) of dried powder formulations of the mentioned mushroom species (APF and LPF, respectively) were evaluated. Powder formulations of both species revealed the presence of essential nutrients, such as proteins, carbohydrates and unsaturated fatty acids. Furthermore, they present a low fat content (<2g/100g) and can be used in low-calorie diets, just like the mushrooms fruiting bodies. APF showed higher antioxidant activity and higher content of tocopherols and phenolic compounds (124 and 770mug/100g, respectively) than LPF (32 and 690mug/100g). Both formulations could be used as antioxidant sources to prevent diseases related to oxidative stress.