{Reference Type}: Journal Article
{Author}: Angelini, P.; Pagiotti, R.; Venanzoni, R.; Granetti, B.
{Year}: 2009
{Title}: Antifungal and allelopathic effects of Asafoetida against Trichoderma harzianum and Pleurotus spp.
{Tag}: 0
{Star}: 0
{Journal}: ALLELOPATHY JOURNAL
{Volume}: 23
{Issue}: 2
{Pages}: 357-367
{ISBN/ISSN}: 0971-4693
{Keywords}: ANTIMICROBIAL ACTIVITY; FERULIC ACID; CARCINOGENESIS; INHIBITION; PROFILES; ENZYMES; FUNGI; Antagonism; GC/MS; green moulds; MIC; MFC; transmission electron microscopy
{Abstract}: Methanol extract (MeOH) of Asafoetida oleogum-resin was assayed for its in-vitro ability to control Trichoderma harzianum. The thirty-two components of MeOH-extracted resin were identified by GC-MS analysis. The antifungal and allelopathic effects of the MeOH extracts concentrations against T. harzianum and Pleurouts spp., were investigated in dual culture experiments on an agar-based medium. MeOH extract showed fungistatic and fungicidal properties against T harzianum strains and Pleurotus spp. at higher concentrations. In dual culture, all strains of T. harzianum were antagonistic to Pleurotus spp. than in control. When MeOH extracts concentrations was added to the substrate culture, the antagonistic activity of T harzianum against the Pleurotus spp. was moderate (0.625 mu g/ml of MeOH extract) or weak (1.25 mu g/ml of MeOH extract) against the Pleurotus spp. that either completely or partially replaced T harzianum. TEM observations revealed that fungal growth inhibition from the MeOH extract was accompanied by marked morphological and cytological changes.
{Author Address}: Dipartimento Biol Applicata, I-06121 Perugia, Italy; Dipartimento Biol Applicata, I-06121 Perugia, Italy; Dipartimento Biol Applicata, I-06121 Perugia, Italy; Dipartimento Biol Applicata, I-06121 Perugia, Italy
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Italy

{Reference Type}: Journal Article
{Author}: Wu, M. F.; Li, P. C.; Chen, C. C.; Ye, S. S.; Chien, C. T.; Yu, C. C.
{Year}: 2011
{Title}: Cordyceps sobolifera extract ameliorates lipopolysaccharide-induced renal dysfunction in the rat
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=21598419&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Am J Chin Med
{Volume}: 39
{Issue}: 3
{Pages}: 523-35
{DOI}: 10.1142/S0192415X11009007
{Date Displayed}: 2011
{Date}: 2011-01-20
{Type of Work}: Journal Article; Research Support, Non-U.S. Gov't
{Accession Number}: 21598419
{Keywords}: Animals; Antioxidants/pharmacology/*therapeutic use; Apoptosis/drug effects; Autophagy/drug effects; Biological Agents/pharmacology/*therapeutic use; Blood Urea Nitrogen; *Cordyceps; Creatinine/blood; Dietary Supplements; Dogs; Epithelial Cells/drug effects; Female; Kidney/blood supply/*drug effects/metabolism; Kidney Diseases/chemically induced/*drug therapy/metabolism; Kidney Tubules/drug effects; Leukocytes/metabolism; Lipopolysaccharides; Oxidative Stress/drug effects; Rats; Rats, Wistar; Swine
{Abstract}: Cordyceps Sobolifera (CS), an economic traditional Chinese herb, may ameliorate nephrotoxicity-induced renal dysfunction in the rat via antioxidant, anti-apoptosis, and anti-autophagy mechanisms. We investigated the water extract   of fermented whole broth of CS on lipopolysaccharide (LPS)-induced renal cell injury in vitro and in vivo. CS effect on LPS-induced epithelial Lilly pork kidney (PK1) and Madin-Darby canine kidney epithelial (MDCK) cell death was detected with MTT assay. Two-month treatment of CS effects on renal blood flow (RBF), glomerular filtration rate (GFR), plasma blood urea nitrogen, creatinine level and leukocytes (WBC) count were determined in the LPS-treated rats. We further examined the effects of CS supplement on renal tubular oxidative stress,   endoplasmic reticulum stress, apoptosis and autophagy by Western blot analysis. LPS dose-dependently induced PK1 and MDCK cell death, which can be ameliorated by CS treatment. LPS significantly decreased RBF and GFR and increased blood leukocyte counts, plasma blood urea nitrogen and creatinine level in the rat after 24 hours of injury. LPS enhanced renal tubular ER stress, autophagy and apoptosis via by increase protein expressions of GRP78, caspase 12, Beclin-1 and   Bax/Bcl-2 ratio. These findings are associated with the significant staining in renal proximal and distal tubular ED-1, GRP78, Beclin-1 autophagy, and TUNEL apoptosis in the LPS-treated kidneys. Two months of CS supplement significantly improved RBF, GFR and WBC values and reduced ED-1, GRP78, Beclin-1 autophagy and   TUNEL apoptosis in the LPS-treated kidneys. Long-term CS treatment reduced LPS-induced stress responses and tissue damage possibly via blocking LPS-triggered signaling pathways.
{Author Address}: Department of Internal Medicine, Kuang-Tien General Hospital, Taichung, Taiwan.
{Language}: eng

{Reference Type}: Journal Article
{Author}: Zhang, G.; Zeng, X.; Li, C.; Li, J.; Huang, Y.; Han, L.; Wei, J. A.; Huang, H.
{Year}: 2011
{Title}: Inhibition of urinary bladder carcinogenesis by aqueous extract of sclerotia of Polyporus umbellatus fries and polyporus polysaccharide
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=21213404&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Am J Chin Med
{Volume}: 39
{Issue}: 1
{Pages}: 135-44
{DOI}: 10.1142/S0192415X11008701
{Date Displayed}: 2011
{Date}: 2011-01-20
{Type of Work}: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
{Accession Number}: 21213404
{Keywords}: Animals; Anticarcinogenic Agents/pharmacology/*therapeutic use; Biological Agents/pharmacology/therapeutic use; Disease Models, Animal; Female; Fungal Structures; Glutathione S-Transferase pi/genetics/*metabolism; NAD(P)H Dehydrogenase (Quinone)/genetics/*metabolism; *Phytotherapy; Polyporus/*chemistry; Polysaccharides/pharmacology/*therapeutic use; RNA, Messenger/metabolism; Rats; Rats, Inbred F344; Up-Regulation; Urinary Bladder Neoplasms/chemically induced/metabolism/*prevention & control
{Abstract}: The study aimed to evaluate inhibition effect of sclerotia of Polyporus umbellatus Fries aqueous extract (SPUE) and polyporus polysaccharide (PPS) on bladder cancer, then to measure their effect on mRNA expression of glutathione S-transferase pi (GSTPi) and NAD(P)H:quinone oxidoreductase 1 (NQO1) in female Fischer-344 rats model. The model rats were induced by N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN) for a period of 8 weeks and saccharin for 12 weeks. SPUE (50 mg/kg, 250 mg/kg, 500 mg/kg) and PPS (28 mg/kg)   were orally administrated to the model rats during the whole study. Compared to the control group, a more preventive effect of SPUE and PPS treatment on bladder   cancer was discovered, higher mRNA upregulation of GSTpi and NQO1 was seen in the treatment group. Furthermore, the GSTPi and NQO1 mRNA upregulated level in the low-dose group (SPUE 50 mg/kg) was at maximum. In brief, SPUE and PPS are highly   effective in inhibiting bladder carcinogenesis in rats, which may be associated with upregulation of GSTPi and NQO1 in the bladder.
{Author Address}: Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, P. R. China.
{Language}: eng

{Reference Type}: Journal Article
{Author}: Li, X. T.; Li, H. C.; Li, C. B.; Dou, D. Q.; Gao, M. B.
{Year}: 2010
{Title}: Protective effects on mitochondria and anti-aging activity of polysaccharides from cultivated fruiting bodies of Cordyceps militaris
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=21061463&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Am J Chin Med
{Volume}: 38
{Issue}: 6
{Pages}: 1093-106
{DOI}: 10.1142/S0192415X10008494
{Date Displayed}: 2010
{Date}: 2010-01-20
{Type of Work}: Journal Article; Research Support, Non-U.S. Gov't
{Accession Number}: 21061463
{Keywords}: Aging/*drug effects/physiology; Animals; Antioxidants/metabolism/pharmacology/*therapeutic use; Cordyceps/*chemistry; Cysteine; Disease Models, Animal; Dose-Response Relationship, Drug; Fruiting Bodies, Fungal; Galactose; Hydroxyl Radical/metabolism; Injections, Subcutaneous; Liver/*drug effects/metabolism; Male; Mice; Mice, Inbred Strains; Mitochondria/*drug effects/metabolism/pathology; Mitochondrial Diseases/*drug therapy/metabolism/pathology; Phytotherapy; Polysaccharides/pharmacology/*therapeutic use; Reactive Oxygen Species/metabolism; Superoxides/metabolism; Thiobarbituric Acid Reactive Substances
{Abstract}: Cordyceps militaris (L.) Link is an entomopathogenic fungus parasitic to Lepidoptera larvae, and is widely used as a folk tonic or invigorant for longevity in China. Although C. militaris has been used in traditional Chinese medicine for millennia, there is still a lack convincing evidence for its anti-aging activities. This study was performed to investigate the effects of polysaccharides from cultivated fruiting bodies of C. militaris (CMP) on mitochondrial injury, antioxidation and anti-aging activity. Fruiting bodies of C. militaris were cultivated artificially under optimized conditions. The spectrophotometric method was used to measure thiobarbituric acid reactive substances (TBARS), mitochondrial swelling, and activities of scavenging superoxide anions in vitro. D-galactose (100 mg/kg/day) was injected subcutaneously into back of the neck of mice for 7 weeks to induce an aging model. The effects of CMP on the activities of catalase (CAT), surperoxide dismutase (SOD), glutathione peroxidase (GPx) and anti-hydroxyl radicals were assayed in vivo using commercial monitoring kits. The results showed that CMP could inhibit mitochondrial injury and swelling induced by Fe(2)(+)-L-Cysteine in a concentration- dependent manner and it also had a significant superoxide anion   scavenging effect. Moreover, the activities of CAT, SOD, GPx and anti-hydroxyl radicals in mice liver were increased significantly by CMP. These results indicate that CMP protects mitochondria by scavenging reactive oxygen species (ROS), inhibiting mitochondrial swelling, and increasing the activities of antioxidases. Therefore, CMP may have pharmaceutical values for mitochondrial protection and anti-aging. CMP was the major bioactive component in C. militaris.
{Author Address}: Dalian Nationalities University, China. xtli@dlnu.edu.cn
{Language}: eng