{Reference Type}: Journal Article
{Author}: Adebayo-Tayo, B. C.; Jonathan, S. G.; Popoola, O. O.; Egbomuche, R. C.
{Year}: 2011
{Title}: Optimization of growth conditions for mycelial yield and exopolysaccharride production by Pleurotus ostreatus cultivated in Nigeria
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
{Volume}: 5
{Issue}: 15
{Pages}: 2130-2138
{ISBN/ISSN}: 1996-0808
{Keywords}: POLYSACCHARIDE PRODUCTION; SUBMERGED CULTURE; EDIBLE MUSHROOM; BIOMASS; PH; Culture; Pleurotus ostreatus; mycelia yield; exopolysaccharride-production
{Abstract}: Optimum culture condition for mycelia and exopolysaccharides production (EPS) by Pleurotus ostreatus in submerged liquid culture was investigated. The optimum temperature for mycelia yield and exopolysaccharide production was 25 and 15 degrees C in day 14 with yields of 1.40 g/ml and 2700 mg/l respectively. Optimum pH was found to be 8 with respective yields of 2.5 g/ml and 2665 mg/l in day 12 for mycelia and EPS mannitol (9.75 g/litre) and raffinose stimulated the highest mycelia growth (3.0 g/ml) and EPS production (3000 mg/l) at day 14 respectively. Mycelia yield (2.10 g/ml) and EPS production (2700 mg/l) was optimally supported by urea. Glycine (1.0 g) and leucine (1.0 g) supported optimum production of mycelia yield (2.5 g/ml) and EPS concentration (2925 mg/l) respectively while folic acid and ascorbic acid induced the moderate mycelia yield and EPS concentration respectively. These results have shown that significant improvement in mycelia yield and EPS production by P. ostreatus could be enhanced through submerged cultivation under appropriate optimized conditions.
{Author Address}: Univ Ibadan, Dept Bot & Microbiol, Ibadan, Oyo State, Nigeria; Univ Ibadan, Dept Bot & Microbiol, Ibadan, Oyo State, Nigeria; Univ Ibadan, Dept Bot & Microbiol, Ibadan, Oyo State, Nigeria; Univ Uyo, Dept Microbiol, Uyo, Akwa Ibom State, Nigeria
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Nigeria; Nigeria

{Reference Type}: Journal Article
{Author}: Liu, H. G.; Li, T.; Zhao, Y. L.; Zhang, J.; Wang, Y. Z.
{Year}: 2011
{Title}: Determination of some metabolites of Cordyceps sobolifera
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
{Volume}: 5
{Issue}: 30
{Pages}: 5518-5522
{ISBN/ISSN}: 1996-0808
{Keywords}: TRADITIONAL CHINESE MEDICINE; SCIENTIFIC REDISCOVERY; SINENSIS; Cordyceps sobolifera; mannitol; polysaccharide; total saponins; total flavonoids
{Abstract}: Cordyceps sobolifera is an edible Chinese native medicinal material. Profiles of mannitol, polysaccharides, total saponins and total flavonoids in 12 populations of C. sobolifera were analyzed to evaluate the characteristics of this species. The amount of mannitol was uniformly distributed among the populations, which ranged from 5.78 to 10.91 g/100 g. The highest point reached by polysaccharide was 2.27 g/100 g and the lowest point was 0.35 g/100 g. It was the first report on content of total saponins in C. sobolifera and it was found not to be so rich in C. sobolifera. The highest amount of total flavonoids was 1.28 g/100 g. Mannitol was higher in stroma (11.37 g/100 g) than the one in sclerotium (6.42 g/100 g). It suggested that C. sobolifera might be a good source of mannitol. However, polysaccharide, total saponins and total flavonoids contents in the stroma were not different from those in the sclerotium.
{Author Address}: Yunnan Agr Univ, Kunming 650201, Yunnan, Peoples R China; Yuxi Normal Univ, Yuxi 653100, Yunnan, Peoples R China; Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650223, Yunnan, Peoples R China; Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650223, Yunnan, Peoples R China; Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650223, Yunnan, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Wu, Fengyao; Yan, Hui; Ma, Xiaoning; Jia, Junqing; Zhang, Guozheng; Guo, Xijie; Gui, Zhongzheng
{Year}: 2011
{Title}: Structural characterization and antioxidant activity of purified polysaccharide from cultured Cordyceps militaris
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
{Volume}: 5
{Issue}: 18
{Pages}: 2743-2751
{ISBN/ISSN}: 1996-0808
{Keywords}: RADICAL SCAVENGING ACTIVITIES; IN-VITRO; LIPID-PEROXIDATION; SINENSIS; IDENTIFICATION; ANTITUMOR; MYCELIA; EXTRACT
{Abstract}: The polysaccharide fraction, CM-hs-CPS2, was isolated from fruiting bodies of cultured Cordyceps militaris grown on solid rice medium by hot water extraction and ethanol precipitation, and purified by ion exchange column (DEAE-cellulose-52) and gel filtration column (Sephadex G-100) chromatography. Its structural characterizations were analyzed by gas chromatograph (GC) and fourier transform infrared spectroscopy (FT-IR). Results indicated that CM-hs-CPS2 was a kind of sulfating acidic polysaccharide containing acetamido group. The monosaccharide composition analysis showed that CM-hs-CPS2 was composed mainly of mannose, glucose and galactose in a molar ration of 1.35: 8.34: 1.00, and linked by a-glycosidic linkage. The studies on antioxidant activities of CM-hs-CPS2 were evaluated by various methods in vitro. Results showed that DPPH. scavenging activity, reducing power and ferrous ion chelating activity of CM-hs-CPS2 (8 mg/ml) were 89%, 1.188 and 85%, respectively.
{Author Address}: Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Peoples R China; Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Peoples R China; Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Peoples R China; Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Bolla, Krishna; Shaheen, Syed Zeenat; Vasu, Kandukuri; Charya, M. A. Singara
{Year}: 2008
{Title}: Effect of oils on the production of exopolysaccharides and mycelial biomass in submerged culture of Schizophyllum commune
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
{Volume}: 2
{Issue}: 12
{Pages}: 349-352
{ISBN/ISSN}: 1996-0808
{Keywords}: FATTY-ACIDS; Schizophyllum commune; exopolysaccharides; submerged culture; oil
{Abstract}: The effect of oils (olive, castor and peppermint) addition at different concentrations on the cell growth and production of exopolysaccharides (EPS) in a submerged culture of Schizophyllum commune was determined. The highest cell growth (8.2 g. dr.w/l) was observed on the 14(th) day of incubation in medium containing 1% of the olive oil. EPS production was slightly enhanced by olive oil and castor oil, but significantly inhibited by peppermint oil. Mycelial growth was increased with the oils used at different concentrations, except 1% at 7 and 14 days of incubation. Amongst the three oil sources examined, the highest EPS production was observed in medium with 0.5% castor oil after 7 days of incubation.
{Author Address}: Kakatiya Univ, Dept Microbiol, Warangal 506009, Andhra Pradesh, India; Kakatiya Univ, Dept Microbiol, Warangal 506009, Andhra Pradesh, India; Kakatiya Univ, Dept Microbiol, Warangal 506009, Andhra Pradesh, India; Kakatiya Univ, Dept Microbiol, Warangal 506009, Andhra Pradesh, India
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: India