{Reference Type}: Journal Article
{Author}: Ding, Zhongyang; Peng, Lin; Chen, Youzhi; Zhang, Liang; ZhenghuaGu; Shi, Guiyang; Zhang, Kechang
{Year}: 2012
{Title}: Production and characterization of thermostable laccase from the mushroom, Ganoderma lucidum, using submerged fermentation
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
{Volume}: 6
{Issue}: 6
{Pages}: 1147-1157
{ISBN/ISSN}: 1996-0808
{Keywords}: MANGANESE PEROXIDASE; TRAMETES-VERSICOLOR; PLEUROTUS-OSTREATUS; PURIFICATION; DECOLORIZATION; BASIDIOMYCETE; CULTURES; ENZYMES; LIGNIN; WASTES; Laccase; Ganoderma lucidum; submerged fermentation; pomelo peel
{Abstract}: Laccase production by the white rot fungus, Ganoderma lucidum, was investigated using submerged fermentation, and the laccase was purified and characterized. The effects of variations in the media nutrient composition or culture conditions and characteristics of purified laccase were provided for large scale production or industrial uses of the laccase. Low concentrations of glucose and yeast extract stimulated G. lucidum laccase production. The addition of lignocellulosic substrates to the media stimulated laccase production: pomelo peel induced the highest laccase activity of 11842.13 U/L and banana peel reduced laccase production, compared to control media containing wheat bran. Native PAGE and SDS PAGE demonstrated the presence of a single isoform with a molecular weight of 68 kDa. The optimum pH and temperature were 3.0 and 60 degrees C, respectively. G. lucidum laccase is thermostable, with a residual activity of 46% after 80 min at 60 degrees C. The Michaelis-Menten constant (K-m) and catalytic constant (K-cat) values for G. lucidum laccase using ABTS (2,2'-azino-di-[3-ethyl-benzothiazolinsulphonate]) as a substrate were 0.114 mM and 74.63 S-1, respectively, with a specificity constant (K-cat/K-m) of 654.65 S-1 mM(-1). The thermostable properties of the laccase produced by fermentation of G. lucidum in submerged culture had potential for industrial and biotechnology applications.
{Author Address}: Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Daba, Ayman S.; Youssef, Ghada A.; Kabeil, Sanaa S.; Hafez, Elsayed E.
{Year}: 2011
{Title}: Production of recombinant cellulase enzyme from Pleurotus ostreatus (Jacq.) P. Kumm. (type NRRL-0366)
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
{Volume}: 5
{Issue}: 10
{Pages}: 1197-1202
{ISBN/ISSN}: 1996-0808
{Keywords}: BIOTECHNOLOGY; PROTEINS; Pleurotus ostreatus; mushroom; avicel; recombinant cellulolytic enzymes
{Abstract}: Successful utilization of Pleurotus ostreatus (Jacq.) P. Kumm. (type NRRL-0366) mushroom as a type of edible locally isolated mushroom in Egypt at the Mushroom Research Center (Mubarak City for Scientific Research and Technology Applications), to produce extensive hydrolyzing cellulase complex enzymes. This hydrolysis was approached in submerged culture supplemented with avicel PH101 as a substrate for endo-, exoglucanase production. The avicel concentration 6% yielded the maximum enzyme activities (2.46, 1.80 U/ml) for both endo-and exoglucanase activities on basal medium at 27 degrees C, initial pH value of 5.5 for 12 days on rotary shaker (180 rpm) incubation period. Cellulase enzyme was amplified using specific PCR and the amplicone was cloned using TOPO TA cloning vector. The cellulolytic activity of the recombinant protein was examined and high activity was obtained compared with the standard ones. The avicel was used as a sole carbon source in the fermentation medium and the results revealed that, avicel induced the cellulolytic activity of the examined organism compared with those grown on medium deficient of avicel.
{Author Address}: Mubarak City Sci Res & Technol Applicat, Alexandria, Egypt; Univ Alexandria, Fac Sci, Dept Bot & Microbiol, Alexandria, Egypt; Mubarak City Sci Res & Technol Applicat, Alexandria, Egypt; Mubarak City Sci Res & Technol Applicat, Alexandria, Egypt
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Egypt; Egypt

{Reference Type}: Journal Article
{Author}: Goyal, Meenakshi; Soni, Giridhar
{Year}: 2011
{Title}: Production and characterization of cellulolytic enzymes by Pleurotus florida
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
{Volume}: 5
{Issue}: 10
{Pages}: 1131-1136
{ISBN/ISSN}: 1996-0808
{Keywords}: WHEAT-STRAW; LIGNOCELLULOSE; MUSHROOM; Endoglucanase; exoglucanase; fermentation conditions; beta-glucosidase; medium composition
{Abstract}: Three Pleurotus spp., Pleurotus florida, Pleurotus ostreatus and Pleurotus sajar-caju, were screened for cellulolytic enzyme production under submerged fermentation conditions. Of these, P. florida, was studied for optimizing medium composition, incubation period, initial pH and incubation temperature to maximize cellulolytic enzyme production. Malt extract at 0.5%, 12 day of incubation period and 1% CMC as carbon source supported maximum production of cellulases. The optimum temperature and pH for maximum production of enzymes were 35 to 40 degrees C and 5.0 for exo-and endoglucanases and 30 degrees C and 4.5 for beta-glucosidase.
{Author Address}: Punjab Agr Univ, Dept Biochem, Ludhiana 141004, Punjab, India; Punjab Agr Univ, Dept Biochem, Ludhiana 141004, Punjab, India
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: India

{Reference Type}: Journal Article
{Author}: Biyik, Halil; Kalyoncu, Fatih; Oryasin, Erman; Azbar, Nuri; Kalmis, Erbil; Basbulbul, Gamze
{Year}: 2009
{Title}: Evaluation of wild and commercial types of Pleurotus strains for their ability to decolorize cibacron black W-NN textile dye
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
{Volume}: 3
{Issue}: 6
{Pages}: 325-329
{ISBN/ISSN}: 1996-0808
{Keywords}: WHITE-ROT FUNGI; INDUSTRIAL DYES; AZO DYES; OSTREATUS; ENZYMES; BIODEGRADATION; EFFLUENTS; OXIDATION; DJAMOR; GROWTH; Agar plate screening; biological decolorisation; Pleurotus spp.; textile dye
{Abstract}: Biological decolorisation of Cibacron Black W-NN, was comparatively studied using five commercial (Pleurotus djamor, P. ostreatus, P. citrinopileatus, P. eryngii and P. sajor-caju) and three wild types of (Pleurotus ostreatus, P. ostreatus and P. eryngii) white rot fungi strains on agar plates. The initial dye concentrations in the medium were 20, 50, 100, 200 and 500 mg/L, respectively. All P. ostreatus strains and P. sajor-caju fully decolorized Cibacron Black W-NN without any problem. Other organisms were found to be negatively affected from increasing dye concentrations and were able to decolorize the dye used up to a certain concentration (50 mg/L for P. citrinopileatus; 100 mg/L for commercial P. eryngii and 200 mg/L for wild P. eryngii).
{Author Address}: Adnan Menderes Univ, Fac Sci & Arts, Dept Biol, Aydin, Turkey; Celal Bayar Univ, Fac Sci & Arts, Dept Biol, Manisa, Turkey; Adnan Menderes Univ, Fac Sci & Arts, Dept Biol, Aydin, Turkey; Ege Univ, Fac Engn, Dept Bioengn, Izmir, Turkey; Ege Univ, Fac Engn, Dept Bioengn, Izmir, Turkey; Adnan Menderes Univ, Fac Sci & Arts, Dept Biol, Aydin, Turkey
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Turkey; Turkey; Turkey