{Reference Type}: Journal Article
{Author}: Yan, Hui; Zhu, Dongjie; Xu, Dabao; Wu, Jie; Bian, Xuyun
{Year}: 2008
{Title}: A study on Cordyceps militaris polysaccharide purification, composition and activity analysis
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 7
{Issue}: 22
{Pages}: 4004-4009
{ISBN/ISSN}: 1684-5315
{Keywords}: INDUCED LIVER-INJURY; BIOLOGICAL-ACTIVITIES; RATS; Cordyceps militaris polysaccharide; gas chromatography; hepatocyte injury; anti-oxidation
{Abstract}: In this study, raw Cordyceps militaris polysaccharide (CPS) was extracted with ultrasonic-assisted extraction method. Proteins in the raw CPS were removed with the enzymatic and Sevag methods. After dialysis, an extraction rate of 0.82% was achieved. Two components, namely CPS-1 and CPS-2, were obtained from DEAE-52 ionic exchange chromatography eluted with NaCl solution at a gradient from 0 to 0.6 mol.L-1. The ultraviolet scanning demonstrated that CPS-1 did not couple with protein and nucleic acid, but CPS-2 was coupled with a few proteins and nucleic acids. After product derivatization using acid hydrolysis, gas chromatography analysis revealed that these two CPSs were both composed of mannose and galactose, with a mole ratio of 0.96:1 and 1.04:1, respectively. The two CPSs can reverse ethanol-induced injury in primary cultured rat hepatocytes. Its effect appeared dose-dependent, having better effect when higher dose was applied. Its effect is probably due to its anti-oxidation function.
{Author Address}: Jiangsu Univ Sci & Technol, Sch Biol & Environm Engn, Zhenjiang 212018, Jiangsu, Peoples R China; Jiangsu Univ Sci & Technol, Sch Biol & Environm Engn, Zhenjiang 212018, Jiangsu, Peoples R China; Jiangsu Univ Sci & Technol, Sch Biol & Environm Engn, Zhenjiang 212018, Jiangsu, Peoples R China; Jiangsu Univ Sci & Technol, Sch Biol & Environm Engn, Zhenjiang 212018, Jiangsu, Peoples R China; Jiangsu Univ Sci & Technol, Sch Biol & Environm Engn, Zhenjiang 212018, Jiangsu, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China

{Reference Type}: Journal Article
{Author}: Selvi, S.; Umadevi, P.; Murugan, S.; Senapathy, Giftson J.
{Year}: 2011
{Title}: Anticancer potential evoked by Pleurotus florida and Calocybe indica using T-24 urinary bladder cancer cell line
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 10
{Issue}: 37
{Pages}: 7279-7285
{ISBN/ISSN}: 1684-5315
{Keywords}: MEDICINAL MUSHROOMS; APOPTOSIS; AGENTS; ANTITUMOR; POLYSACCHARIDES; ANTIOXIDANT; EXTRACTION; OSTREATUS; THERAPY; Bladder cancer; MTT; DNA fragmentation; mushroom; antitumour
{Abstract}: Cancer is the second largest non-communicable disease and it has a sizable contribution in the total number of deaths. Cancer of the urinary bladder ranks fourth in men and eighth in women with an alarming increase in cancer patients of this type every year. Tobacco smoking is considered to be one of the causes of bladder cancer and a number of organic chemicals used in the industry are known to be carcinogenic to the bladder. Chemical and dyestuff manufacturers are at particularly high risk of bladder cancer. Edible fungi are used as potential therapeutic agents for the treatment of cancers. This study was employed to find out the therapeutic potential of two medicinal mushroom varieties namely Pleurotus florida and Calocybe indica against T24 urinary bladder cancer cell lines. The therapeutic potential of the two medicinal mushroom varieties, P. florida and C. indica against T24 urinary bladder cancer cell lines were determined by the MTT assay and DNA fragmentation assay. The results obtained from the MTT assay and DNA fragmentation assay in this study showed the anti-tumour potential of these two mushroom varieties against T24 bladder cancer cell lines. Thus, the mushroom varieties P. florida and C. indica can be employed as potential anticancer drugs against bladder carcinoma.
{Author Address}: Bharathidasan Coll Arts & Sci Erode, Dept Biochem, Erode, Tamil Nadu, India; Karunya Univ, Dept Biotechnol, Sch Biotechnol & Hlth Sci, Coimbatore 641114, Tamil Nadu, India; Karunya Univ, Dept Biotechnol, Sch Biotechnol & Hlth Sci, Coimbatore 641114, Tamil Nadu, India; Karunya Univ, Dept Biotechnol, Sch Biotechnol & Hlth Sci, Coimbatore 641114, Tamil Nadu, India
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: India; India

{Reference Type}: Journal Article
{Author}: Lung, Ming-Yeou; Chang, Yu-Cheng
{Year}: 2011
{Title}: In vitro antioxidant properties of polysaccharides from Armillaria mellea in batch fermentation
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 10
{Issue}: 36
{Number}: WOS:000294668500030
{Pages}: 7048-7057
{Cited Count}: 2
{Date Displayed}: 2011
JUL 18 2011
{ISBN/ISSN}: 1684-5315
{Abstract}: Antioxidant properties of exopolysaccharides (EPS) and intracellular polysaccharides (IPS) obtained respectively from mycelia and filtrates of submerged culture by Armillaria mellea in a 20-L stirred tank bioreactor were investigated. Effective production of EPS and IPS is available by submerged culture of A. mellea with respective number average molecular weights and protein/polysaccharide ratios as 7.68x10(6) Da and 7.68% as well as 5.65x10(6) Da and 5.26%. Both EPS and IPS exhibit powerful antioxidant activities by conjugated diene method, chelating effect on ferrous ions and scavenging effect on superoxide anion, as evidenced by their quite low EC(50) values (< 5 mg/mL). Results confirmed that fermented A. mellea polysaccharides are potential antioxidant sources of both healthy medicine and food industries.

{Reference Type}: Journal Article
{Author}: Xiao, Jian-Hui; Xiao, Dai-Min; Sun, Zhong-Hua; Xiao, Yu; Zhong, Jian-Jiang
{Year}: 2011
{Title}: Antioxidative potential of polysaccharide fractions produced from traditional Chinese medicinal macrofungus Cordyceps jiangxiensis in vitro
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 10
{Issue}: 34
{Pages}: 6607-6615
{ISBN/ISSN}: 1684-5315
{Keywords}: SINENSIS; MYCELIA; EXTRACTS; MILITARIS; ARREST; GROWTH; CELLS; Cordyceps jiangxiensis; polysaccharide; antioxidant activity
{Abstract}: Cordyceps jiangxiensis, also called 'CaoMuWang', is a medicinal entomopathogenic macrofungus native to eastern China. Polysaccharide fractions from cultured C. jiangxiensis exhibited potent antitumor activity via the induction of cell cycle arrest and apoptotic pathway. Antioxidant pathway is also one action of mechanism of antitumor; thus, the antioxidant abilities of these polysaccharide fractions were overall evaluated by five in vitro assays such as the scavenging abilities on DPPH center dot, hydroxyl and superoxide anion radicals, the reducing power and the chelating ability on ferrous ions. Among these assays, the polysaccharide fractions presented more excellent scavenging abilities on superoxide anion radicals than that of the positive control. When compared with the positive control, the polysaccharide fractions from C. jiangxiensis only had moderate scavenging activities on both DPPH and hydroxyl free radicals, moderate reducing power and ferrous ion chelating activity. The antioxidant abilities of the different polysaccharide fractions had certain differences at all the tested doses and all had a dose-dependent manner. The results suggested that, polysaccharides are important antioxidant component in the medicinal Cordyceps fungi and have direct and potent antioxidant ability, and that C. jiangxiensis also is a promising potential source for the development of natural antioxidant.
{Author Address}: Zunyi Med Coll, Div Appl Mycol &amp; Biochem Pharm, Key Lab Cell Engn Guizhou Prov, Affiliated Hosp, Zunyi 563000, Peoples R China; E China Univ Sci &amp; Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China; Zunyi Med Coll, Div Appl Mycol &amp; Biochem Pharm, Key Lab Cell Engn Guizhou Prov, Affiliated Hosp, Zunyi 563000, Peoples R China; Zunyi Med Coll, Div Appl Mycol &amp; Biochem Pharm, Key Lab Cell Engn Guizhou Prov, Affiliated Hosp, Zunyi 563000, Peoples R China; Zunyi Med Coll, Div Appl Mycol &amp; Biochem Pharm, Key Lab Cell Engn Guizhou Prov, Affiliated Hosp, Zunyi 563000, Peoples R China; E China Univ Sci &amp; Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China; Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China; Peoples R China