{Reference Type}: Journal Article
{Author}: Zheng, Li Ping; Gao, Li Wen; Zhou, Jian Qin; Sima, Yang Hu; Wang, Jian Wen
{Year}: 2008
{Title}: Antioxidant activity of aqueous extract of a Tolypocladium sp fungus isolated from wild Cordyceps sinensis
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 7
{Issue}: 17
{Pages}: 3004-3010
{ISBN/ISSN}: 1684-5315
{Keywords}: SCIENTIFIC REDISCOVERY; IN-VITRO; EXOPOLYSACCHARIDE; POLYPHENOLS; MILITARIS; INONOTUS; RADICALS; CELLS; EPS2; Aqueous extracts; antioxidant activity; free radicals; PC12 cells; Tolypocladium sp.
{Abstract}: Tolypocladium sp. Ts-1 was isolated from the fruiting body of a wild Cordyceps sinensis, one of the best known traditional Chinese medicine and health foods. The antioxidant activities of hot-water extracts from cultured mycelia of Tolypocladium sp. were assessed in different in vitro systems. The extracts showed superoxide dismutase (SOD) activity of 35.6 U/mg protein and are effective in scavenging superoxide radical in a concentration dependent fashion with IC50 value of 1.3 mg/mL. As a reinforcement of the action, similar radical scavenging effects of the extracts were also discerned with both site-specific and non site-specific hydroxyl radical using the deoxyribose assay method. 1,1-Diphenyl- 2-picrylhydracyl (DPPH) radical scavenging activities of the extracts reached more than 75.2% at the concentrations of 3-6 mg/mL. The extracts showed moderate reducing power and ferrous ion chelating activity. Moreover, the protective effects of the extracts against hydrogen peroxide (H2O2)-induced lesion to rat PC12 cell (pheochromocytoma cell line) was observed in a dose-dependent manner from 0.25 to 2.0 mg/mL. Our results suggest that the aqueous extract of Tolypocladium sp. mycelium has strong antioxidant activities and is a potential source of natural antioxidant products. This is the first report on antioxidant activity of Tolypocladium fungus isolated from wild C. sinensis, an endangered species.
{Author Address}: Soochow Univ, Dept Hort Sci, Suzhou 215123, Peoples R China; Soochow Univ, Sch Pharmaceut Sci, Coll Med, Suzhou 215123, Peoples R China; Soochow Univ, Sch Pharmaceut Sci, Coll Med, Suzhou 215123, Peoples R China; Soochow Univ, Coll Med, Sch Preclin Med & Life Sci, Suzhou 215123, Peoples R China; Soochow Univ, Sch Pharmaceut Sci, Coll Med, Suzhou 215123, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Sun, Zhaogang; Tian, Yu; Jia, Mengshi; Pang, Lifei; Deng, Peng; Fan, Keming; Liu, Xiaonan; Jia, Shouhua; Jia, Le
{Year}: 2012
{Title}: Extraction and in vitro antioxidant activity of exopolysaccharide by Pholiota adiposa SX-01
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
{Volume}: 6
{Issue}: 8
{Pages}: 1869-1876
{ISBN/ISSN}: 1996-0808
{Keywords}: SUBMERGED MYCELIAL CULTURE; OPTIMIZATION; REQUIREMENTS; ANTITUMOR; BIOMASS; Pholiota adiposa SX-01; exopolysaccharide; response surface methodology; antioxidant activity; in vitro
{Abstract}: The extraction parameters for Pholiota adiposa SX-01 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology. The optimum conditions for EPS extraction were predicted to be, concentration at 87.36 degrees C, precipitation for 35.18 h at pH 8.81, and EPS production was estimated to be 8.45 g/L. The actual yield of EPS under these conditions was 8.39 g/L. The in vitro antioxidant activities of the EPS were investigated and results showed that the inhibition effects of EPS at a dosage of 250 mg/L on superoxide anion radical, hydroxyl radical and reducing power were 70.63 +/- 5.26, 58.22 +/- 5.41 and 0.37 +/- 0.02% (absorbance at 700 nm), respectively. The results provide a reference for large-scale extraction of EPS by P. adiopsa SX-01 in industrial fermentation.
{Author Address}: Cent Hosp Taian, Tai An 271000, Shandong, Peoples R China; Ocean Univ China, Qingdao 266003, Shandong, Peoples R China; First High Sch Taian, Tai An 271000, Shandong, Peoples R China; Shandong Agr Univ, Coll Chem & Mat Sci, Tai An 271018, Shandong, Peoples R China; Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China; Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China; Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China; Shandong Agr Univ, Coll Chem & Mat Sci, Tai An 271018, Shandong, Peoples R China; Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China; Peoples R China; Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Wu, Fengyao; Yan, Hui; Ma, Xiaoning; Jia, Junqing; Zhang, Guozheng; Guo, Xijie; Gui, Zhongzheng
{Year}: 2011
{Title}: Structural characterization and antioxidant activity of purified polysaccharide from cultured Cordyceps militaris
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
{Volume}: 5
{Issue}: 18
{Pages}: 2743-2751
{ISBN/ISSN}: 1996-0808
{Keywords}: RADICAL SCAVENGING ACTIVITIES; IN-VITRO; LIPID-PEROXIDATION; SINENSIS; IDENTIFICATION; ANTITUMOR; MYCELIA; EXTRACT
{Abstract}: The polysaccharide fraction, CM-hs-CPS2, was isolated from fruiting bodies of cultured Cordyceps militaris grown on solid rice medium by hot water extraction and ethanol precipitation, and purified by ion exchange column (DEAE-cellulose-52) and gel filtration column (Sephadex G-100) chromatography. Its structural characterizations were analyzed by gas chromatograph (GC) and fourier transform infrared spectroscopy (FT-IR). Results indicated that CM-hs-CPS2 was a kind of sulfating acidic polysaccharide containing acetamido group. The monosaccharide composition analysis showed that CM-hs-CPS2 was composed mainly of mannose, glucose and galactose in a molar ration of 1.35: 8.34: 1.00, and linked by a-glycosidic linkage. The studies on antioxidant activities of CM-hs-CPS2 were evaluated by various methods in vitro. Results showed that DPPH. scavenging activity, reducing power and ferrous ion chelating activity of CM-hs-CPS2 (8 mg/ml) were 89%, 1.188 and 85%, respectively.
{Author Address}: Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Peoples R China; Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Peoples R China; Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Peoples R China; Jiangsu Univ Sci & Technol, Zhenjiang 212018, Peoples R China; Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: QIN, Yao; JIN, Xiao-nan; Park Heui, Dong
{Year}: 2010
{Title}: Comparison of Antioxidant Activities in Black Soybean Preparations Fermented with Various Microorganisms
{URL}: http://www.sciencedirect.com/science/article/pii/S1671292709601917
{Tag}: 0
{Star}: 0
{Journal}: Agricultural Sciences in China
{Volume}: 9
{Issue}: 7
{Pages}: 1065-1071
{Date Displayed}: 2010/7//
{Alternate Title}: Agricultural Sciences in China
{ISBN/ISSN}: 1671-2927
{Keywords}: antioxidant activity; black soybean; microorganisms; fermentation
{Abstract}: Black soybeans [Glycine max (L.) Merrill] were fermented with various GRAS (generally recognized as safe) microorganisms, including Aspergillus sp., Bacillus sp. and yeast (Issatchenkia orientalis) at 30°C for 3 d. The antioxidant contents, including total phenolics and total flavonoids, and the antioxidant activities of 1,1-diphenyl-2-picrylhydrazine (DPPH), ferric reducing antioxidant power (FRAP) and HO.scavenging were analyzed in preparations of fermented black soybeans. Fermented cultures proved to yield significant levels of antioxidants compared with non-fermented cultures (P < 0.05). Fermented black soybean preparations possessed antioxidant activities could be attributed to the total phenolics and flavonoids present.