{Reference Type}: Journal Article
{Author}: Su, Chao-an; Xu, Xiao-yan; Liu, De-yun; Wu, Ming; Zeng, Fan-qing; Zeng, Meng-yao; Wei, Wei; Jiang, Nan; Luo, Xia
{Year}: 2013
{Title}: Isolation and characterization of exopolysaccharide with immunomodulatory activity from fermentation broth of Morchella conica
{Tag}: 0
{Star}: 0
{Journal}: DARU-JOURNAL OF PHARMACEUTICAL SCIENCES
{Volume}: 21
{ISBN/ISSN}: 1560-8115
{Keywords}: GANODERMA-LUCIDUM; IN-VITRO; POLYSACCHARIDES; MACROPHAGE; ROOTS; ANTIOXIDANT; CULTURE; VIVO; Morchella conica; Exopolysaccharides; Submerged liquid culture; Immunomodulatory activity
{Abstract}: Background and the purpose of this study: Mushroom polysaccharides have traditionally been used for the prevention and treatment of a multitude of disorders like infectious illnesses, cancers and various autoimmune diseases. In vitro and in vivo studies suggest that certain polysaccharides affect immune system function. Morchella conica (M. conica) is a species of rare edible mushroom whose multiple medicinal functions have been proven. Thus, the objective of this study is to isolate and characterize of exopolysaccharide from submerged mycelial culture of M. conica, and to evaluate its immunomodulatory activity.
Methods: A water-soluble Morchella conica Polysaccharides (MCP) were extracted and isolated from the fermentation broth of M. conica through a combination of DEAE-cellulose and Sephacryl S-300 HR chromatograph. NMR and IR spectroscopy has played a developing role in identification of polysaccharide with different structure and composition from fungal and plant sources, as well as complex glycosaminoglycans of animal origin. Thus, NMR and IR spectroscopy were used to analyze the chemical structure and composition of the isolated polysaccharide. Moreover, the polysaccharide was tested for its immunomodulatory activity at different concentrations using in vitro model.
Results: The results showed that MCP may significantly modulate nitric oxide production in macrophages, and promote splenocytes proliferation. Analysis from HPLC, infrared spectra and nuclear magnetic resonance spectroscopy showed that MCP was a homogeneous mannan with an average molecular weight of approximately 81.2 kDa. The glycosidic bond links is -> 6)-alpha-D-Man p-(1 ->.
Conclusion: The results suggested that the extracted MCP may modulate nitric oxide production in macrophages and promote splenocytes proliferation, and it may act as a potent immunomodulatory agent.
{Author Address}: Lishui Agr Acad Sci, Lishui 32300, Zhejiang, Peoples R China; Sichuan Acad Chinese Med Sci, Lab Cellular & Mol Biol, Chengdu 610041, Peoples R China; Lishui Agr Acad Sci, Lishui 32300, Zhejiang, Peoples R China; Sichuan Acad Chinese Med Sci, Lab Cellular & Mol Biol, Chengdu 610041, Peoples R China; Lishui Agr Acad Sci, Lishui 32300, Zhejiang, Peoples R China; Sichuan Acad Chinese Med Sci, Lab Cellular & Mol Biol, Chengdu 610041, Peoples R China; Sichuan Acad Chinese Med Sci, Lab Cellular & Mol Biol, Chengdu 610041, Peoples R China; Sichuan Acad Chinese Med Sci, Lab Cellular & Mol Biol, Chengdu 610041, Peoples R China; Sichuan Acad Chinese Med Sci, Lab Cellular & Mol Biol, Chengdu 610041, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Chen, Yan; Hu, Meili; Wang, Cui; Yang, Yuling; Chen, Jianhua; Ding, Jingna; Guo, Wenqiang
{Year}: 2013
{Title}: Characterization and in vitro antitumor activity of polysaccharides from the mycelium of Sarcodon aspratus
{Tag}: 0
{Star}: 0
{Journal}: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
{Volume}: 52
{Pages}: 52-58
{ISBN/ISSN}: 0141-8130
{Keywords}: STRUCTURAL-CHARACTERIZATION; SUBMERGED CULTURE; CELLS; ANTIOXIDANT; FRACTIONS; SEEDS; Sarcodon aspratus; Polysaccharides; Isolation; Chemical analyses; Antitumor activity
{Abstract}: Polysaccharides extracted from mushrooms have shown a variety of medical activities, such as antitumor, immunostimulatory and hypoglycemic activity. In this study, characteristics and the antitumor activities of Sarcodon aspratus polysaccharides were investigated for the possibility of application of S. aspratus in health care and medicine. Two polysaccharide fractions (PSAN and PSAA) were extracted and isolated from the mycelium of S. aspratus. The average molecular weight of PSAN and PSAA were approximately 5.6 x 10(4) Da and 3.83 x 10(5) Da, respectively. PSAN was composed of L-rhamnose, D-xylose and D-mannose, with molar ratios of 1:10:21; PSAA consisted of L-rhamnose, D-xylose, D-mannose, D-glucose and D-galactose, with molar ratios of 1:39:76:10:21. Both PSAN and PSAA presented high antitumor activity against Hela cells in vitro. At a concentration of 400 mg/L and an exposure time of 24 h, the inhibition rates for PSAN and PSAA were 65% and 80%, respectively. PSAN and PSAA exhibited significantly lower cytotoxicity against human normal liver cell line L-02 than Hela tumor cells in comparison with 5-Fu. Polysaccharide extracted from an edible mushroom S. aspratus may be a potential candidate for developing a novel low toxicity antitumor agent. (C) 2012 Elsevier B.V. All rights reserved.
{Author Address}: Anhui Univ, Sch Life Sci, Hefei 230039, Peoples R China; Anhui Univ, Sch Life Sci, Hefei 230039, Peoples R China; Anhui Univ, Sch Life Sci, Hefei 230039, Peoples R China; Anhui Univ, Sch Life Sci, Hefei 230039, Peoples R China; Anhui Univ, Sch Life Sci, Hefei 230039, Peoples R China; Anhui Univ, Sch Life Sci, Hefei 230039, Peoples R China; Anhui Univ, Sch Life Sci, Hefei 230039, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China

{Reference Type}: Journal Article
{Author}: Zhang, Jing; Wang, Haiyan; Xue, Yusheng; Zheng, Qiangsun
{Year}: 2013
{Title}: Cardioprotective and antioxidant activities of a polysaccharide from the root bark of Aralia elata (Miq.) Seem
{URL}: http://www.sciencedirect.com/science/article/pii/S0144861712012714
http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271345&_user=3360835&_pii=S0144861712012714&_check=y&_origin=article&_zone=toolbar&_coverDate=2013--02&view=c&originContentFamily=serial&wchp=dGLzVBA-zSkWA&md5=4518ee901d64c54aa43be9e7ae71dd5e&pid=1-s2.0-S0144861712012714-main.pdf 全文链接
{Tag}: 0
{Star}: 0
{Journal}: Carbohydrate Polymers
{Volume}: 93
{Issue}: 2
{Pages}: 442-448
{Date Displayed}: 2013/4/2/
{Alternate Title}: Carbohydrate Polymers
{ISBN/ISSN}: 0144-8617
{Keywords}: Aralia elata (Miq.) Seem; Cardioprotective; Antioxidant; Polysaccharide; Oxidative stress; H9c2 cardiomyocytes
{Abstract}: A water-soluble polysaccharide (AEP-w1) was isolated from the root bark of Aralia elata and its molecular weight was about 4.5 × 104 Da. Monosaccharide component analysis indicated that AEP-w1 appeared to be arabinogalactan, consisting of arabinose, galactose and trace glucose with molar ratios of 6.3:3.5:0.2. The antioxidant and cardioprotective potential of AEP-w1 were evaluated in vitro for the first time. AEP-w1 showed potent superoxide and hydroxyl radical scavenging activities and reducing power in vitro. Treatment with H2O2 resulted in the death of H9c2 cells, whereas pretreatment with 50–400 μg/ml AEP-w1 for 24 h prior to H2O2 exposure significantly increased cell viability. Furthermore, AEP-w1 evidently suppressed cardiomyocyte apoptosis, the mitochondrial membrane potential change and cytochrome c release in H2O2-treated H9c2 cells. In addition, intracellular reduced glutathione (GSH) reduction caused by H2O2 in H9c2 cells was also restored by AEP-w1 pretreatment. Taken together, these date provided the first evidence that the cardioprotective effects of AEP-w1 in H9c2 cells were at least in part associated with its antioxidant activity and inhibition effect on mitochondrial dysfunction.

{Reference Type}: Journal Article
{Author}: Zhang, Ning; Chen, Haixia; Ma, Lishuai; Zhang, Yu
{Year}: 2013
{Title}: Physical modifications of polysaccharide from Inonotus obliquus and the antioxidant properties
{URL}: http://www.sciencedirect.com/science/article/pii/S0141813012005156
http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271248&_user=3360835&_pii=S0141813012005156&_check=y&_origin=article&_zone=toolbar&_coverDate=2013--31&view=c&originContentFamily=serial&wchp=dGLzVlS-zSkzS&md5=91416680a5c6b22335a0abb94b2e25d4&pid=1-s2.0-S0141813012005156-main.pdf 全文链接
{Tag}: 0
{Star}: 0
{Journal}: International Journal of Biological Macromolecules
{Volume}: 54
{Issue}: 0
{Pages}: 209-215
{Date Displayed}: 2013/3//
{Alternate Title}: International Journal of Biological Macromolecules
{ISBN/ISSN}: 0141-8130
{Keywords}: Inonotus obliquus polysaccharides; Physical modification; Physicochemical properties; Antioxidant activities
{Abstract}: Physical modification of polysaccharides exerted better biological properties because of the change of physicochemical properties. Polysaccharides from Inonotus obliquus (IOPS) were modified by acid, alkali hydrolysis, thermal and ultrasonic treatment in this study. The physicochemical and antioxidant properties of IOPS and its physical modified products were comparatively investigated by chemical methods, gas chromatography, size exclusion chromatography, scanning electron micrograph, circular dichroism spectra, and ferric reducing power assay and lipid peroxidation inhibition assay, respectively. Results showed that physicochemical and antioxidant properties of IOPS were changed after the physical modification of acid, alkali hydrolysis, thermal and ultrasonic treatment. Thermal treated polysaccharide (Th-IOPS) and ultrasonic treated polysaccharide (Ul-IOPS) showed the properties of lower molecular weight distribution, lower intrinsic viscosity, a hyperbranched conformation, and higher antioxidant abilities on ferric-reducing power and lipid peroxidation inhibition activity compared with the native polysaccharide IOPS. Th-IOPS and Ul-IOPS might be explored as a novel potential antioxidant for food industry.