{Reference Type}: Journal Article
{Author}: Chen, X.; Wu, G.; Huang, Z.
{Year}: 2013
{Title}: Structural analysis and antioxidant activities of polysaccharides from cultured Cordyceps militaris
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=23537797&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Int J Biol Macromol
{DOI}: 10.1016/j.ijbiomac.2013.03.041
{Date Displayed}: 2013 Mar 25
{Date}: 2013-03-25
{Type of Work}: JOURNAL ARTICLE
{Original Publication}: Copyright (c) 2013. Published by Elsevier B.V.
{Accession Number}: 23537797
{Abstract}: The polysaccharides extracted by hot water and precipitated by 50% ethanol from the fruiting bodies of cultured Cordyceps militaris (W-CBP50) were introduced. Antioxidant activities of W-CBP50, W-CBP50 I (obtained from W-CBP50 purified by Sephadex G-100 chromatography) and W-CBP50II (obtained from W-CBP50I purified by   Sephadex G-200 chromatography) were evaluated and structural features of W-CBP50IIwere analyzed. The polysaccharide W-CBP50II was able to scavenge the stable free radical 1, 1-diphenyl-2-picrylhyrazyl (DPPH), while W-CBP50 and W-CBP50I could scavenge DPPH, hydroxyl and superoxide radical. Structural characteristics of W-CBP50IIwere investigated using GPC, GC-MS, 1H NMR and IR. The results showed that it was mainly composed of alpha- glucose, alpha-mannose,   alpha-galactose and alpha-arabinose with alpha-type glycosidic linkage and the molecular weight of its main component was 8.97kD.
{Author Address}: Fisheries College, Guangdong Ocean University, Zhanjiang 524088. Electronic address: lilychen77@163.com.
{Language}: ENG

{Reference Type}: Journal Article
{Author}: Gao, C.; Wang, Y.; Wang, C.; Wang, Z.
{Year}: 2013
{Title}: Antioxidant and immunological activity in vitro of polysaccharides from Gomphidius rutilus mycelium
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=23399275&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Carbohydr Polym
{Volume}: 92
{Issue}: 2
{Pages}: 2187-92
{DOI}: 10.1016/j.carbpol.2012.12.011
{Date Displayed}: 2013 Feb 15
{Date}: 2013-02-15
{Type of Work}: Journal Article; Research Support, Non-U.S. Gov't
{Original Publication}: Copyright (c) 2012 Elsevier Ltd. All rights reserved.
{Accession Number}: 23399275
{Abstract}: Two novel polysaccharide fractions, GRMP1 and GRMP2, were isolated from the mycelium of Gomphidius rutilus through submerged fermentation. GRMP1 and GRMP2 had similar average molecular weights (35 and 31 kDa, respectively), and were composed of glucose and xylose at molar ratios of 0.46:1 and 0.63:1, respectively. In vitro antioxidant tests showed that GRMP1 and GRMP2 partly scavenged superoxide radical but almost had no scavenging effect on 1,1-diphenyl-2-picrylhydrazyl free radical. The effect of GRMP1 on hydroxyl radicals was stronger than that of GRMP2. Both GRMP1 and GRMP2 had relatively low reducing power and significant lymphocyte proliferation activity. In the presence of concanavalin A or lipopolysaccharide as mitogens for lymphocytes, the lymphocyte proliferation activity increased for GRMP1 but not for GRMP2 within the test dosage range.
{Author Address}: School of Environmental and Biological Engineering, Liaoning Shihua University, Fushun 113001, PR China.
{Language}: eng

{Reference Type}: Journal Article
{Author}: Han, Z. H.; Ye, J. M.; Wang, G. F.
{Year}: 2013
{Title}: Evaluation of in vivo antioxidant activity of Hericium erinaceus polysaccharides
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=23000690&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Int J Biol Macromol
{Volume}: 52
{Pages}: 66-71
{DOI}: 10.1016/j.ijbiomac.2012.09.009
{Date Displayed}: 2013 Jan
{Date}: 2013-01-01
{Type of Work}: Journal Article
{Original Publication}: Copyright (c) 2012 Elsevier B.V. All rights reserved.
{Accession Number}: 23000690
{Abstract}: Hericium erinaceus polysaccharide (HEP) is a traditional Chinese medicine. In the present study, chemical composition and antioxidant activity of HEP was investigated. HPLC analysis showed that the HEP was composed of xylose (7.8%), ribose (2.7%), glucose (68.4%), arabinose (11.3%), galactose (2.5%) and mannose (5.2%). HEP was pre-administered to mice by gavage at a dose of 300 mg/kg for 15   days. Results found that HEP preadministration resulted in a significant decline   in blood urea nitrogen (BUN), serum creatinine (Scr) and increase in creatinine clearance (CrCI) levels in HEP-pretreated group compared to renal ischemia reperfusion (IR) group. Malondialdehyde (MDA) level significantly increased, whereas Level of reduced glutathione (GSH) markedly decreased in renal IR animals. These results indicate that IR induced renal oxidative injury damage, as indicated by a increase in MDA level, and decrease in GSH level as well as the antioxidant enzymes activity. Such effects reflect that HEP can significantly decrease lipid peroxidation level and increase antioxidant enzymes activities in   experimental animals.
{Author Address}: Department of Urology, Luqiao Hospital of Taizhou Enze Medical Center (Group), Taizhou, Zhejiang Province 318050, PR China.
{Language}: eng

{Reference Type}: Journal Article
{Author}: You, L.; Gao, Q.; Feng, M.; Yang, B.; Ren, J.; Gu, L.; Cui, C.; Zhao, M.
{Year}: 2013
{Title}: Structural characterisation of polysaccharides from Tricholoma matsutake and their antioxidant and antitumour activities
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=23497882&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Food Chem
{Volume}: 138
{Issue}: 4
{Pages}: 2242-9
{DOI}: 10.1016/j.foodchem.2012.11.140
{Date Displayed}: 2013 Jun 15
{Date}: 2013-06-15
{Type of Work}: Journal Article
{Original Publication}: Copyright (c) 2012 Elsevier Ltd. All rights reserved.
{Accession Number}: 23497882
{Abstract}: In this study, polysaccharides from Tricholoma matsutake (TM-P) were purified using a DEAE-Sepharose fast flow column and three polysaccharide fractions (TM-P1, TM-P2 and TM-P3) were obtained. The chemical composition and structural characteristics of TM-P2 were quite different from those of TM-P1 and TM-P3. TM-P2 consisted of glucose, galactose and mannose with a molar ratio of 5.9:1.1:1.0. The glycosidic linkages were mainly composed of 1,6- and 1-linked glucose. Furthermore, TM-P2 showed the strongest in vitro antioxidant and antitumour activities. The oxygen radical absorbance capacity (ORAC) of TM-P2 was 2100.44mumol Trolox/g. The antiproliferative activities of TM-P2 (4.0mg/ml) on the growth of HepG2 and A549 cells were 67.98% and 59.04%, respectively.
{Author Address}: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
{Language}: eng