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第二十二部分
发布时间:2014/6/3  阅读次数:1462  字体大小: 【】 【】【

{Reference Type}: Book
{Author}: Vamanu, Emanuel
{Year}: 2013
{Title}: STUDIES ON THE ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF PLEUROTUS OSTREATUS PSI101109 MYCELIUM
{Tag}: 0
{Star}: 0
{Volume}: 45
{Pages}: 311-317
{ISBN/ISSN}: 0556-3321
{Keywords}: RADICAL SCAVENGING CAPACITY; ETHANOLIC EXTRACT; EDIBLE MUSHROOMS; OYSTER MUSHROOMS; WILD; BIOTECHNOLOGY; ANTICANCER; TURKEY; FRUIT; LEAF
{Abstract}: The antioxidant and antimicrobial potential of the ethanolic extract from Pleurotus ostreatus PSI101109 mycelium was determined based on inorganic and organic nitrogen sources in a culture medium. The presence of ammonium sulphate and corn extract resulted in a greater accumulation of bioactive compounds. The results were confirmed by the EC50 and minimum inhibitory concentration (MIC). The extracts of P. ostreatus PSI101109 mycelium selectively inhibited the strains used, both of which were of the genus Candida, at an MIC value of 12.5 mg ml(-1). The most resistant strains were found to be Escherichia coli and Listeria innocua. The capacity of the antioxidant was evaluated by the DPPH scavenging activity, beta-carotene-linoleic acid, the reducing power, the scavenging effect on superoxide radical, the scavenging effect on hydroxyl radical and the scavenging activity on nitric oxide. Compounds with antioxidant effects were present in the extracts. Lycopene was an exception because it was found to be lacking in values when culturation was performed in the presence of peptone and yeast extract, but had a maximum value on ammonium sulphate.
{Author Address}: USAMV Fac Biotechnol, Bucharest 011464, Romania; Ctr Appl Biochem & Biotechnol Bucharest, Bucharest 011464, Romania
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Romania; Romania


{Reference Type}: Journal Article
{Author}: Aydinoglu, Tugba; Sargin, Sayit
{Year}: 2013
{Title}: Production of laccase from Trametes versicolor by solid-state fermentation using olive leaves as a phenolic substrate
{Tag}: 0
{Star}: 0
{Journal}: BIOPROCESS AND BIOSYSTEMS ENGINEERING
{Volume}: 36
{Issue}: 2
{Pages}: 215-222
{ISBN/ISSN}: 1615-7591
{Keywords}: VITRO ANTIMICROBIAL ACTIVITY; PLEUROTUS-OSTREATUS; ANTIOXIDANT ACTIVITY; FUNGAL LACCASES; WASTE; DECOLORIZATION; HIRSUTA; DYES; BIOSYNTHESIS; PURIFICATION; Laccase; Olive leaves; Olea europaea L.; Trametes versicolor; Solid-state fermentation
{Abstract}: The aim of the present study was to investigate whether olive leaves were feasible as a substrate for laccase production by the white-rot fungus Trametes versicolor FPRL 28A INI under solid-state fermentation conditions. Different experiments were conducted to select the variables that allow obtaining high levels of laccase activity. In particular, the effects of the initial moisture content, substrate particle size, supplementation with inorganic and organic nitrogen sources were evaluated. Highest laccase activity (276.62 +/- A 25.67 U/g dry substrate) was achieved with 80 % initial moisture content and 1.4-1.6 mm particle size of the substrate supplemented with yeast extract (1 % (w/w) nitrogen). Such a high activity was obtained without any addition of inducers.
{Author Address}: Ege Univ, Inst Nat & Appl Sci, TR-35100 Bornova, Turkey; Ege Univ, Fac Engn, Dept Bioengn, TR-35100 Bornova, Turkey
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Turkey; Turkey


{Reference Type}: Journal Article
{Author}: Patra, Sukesh; Patra, Pradip; Maity, Kankan K.; Mandal, Soumitra; Bhunia, Sanjay K.; Dey, Biswajit; Devi, K. Sanjana P.; Khatua, Somanjana; Acharya, Krishnendu; Maiti, Tapas K.; Islam, Syed S.
{Year}: 2013
{Title}: A heteroglycan from the mycelia of Pleurotus ostreatus: structure determination and study of antioxidant properties
{Tag}: 0
{Star}: 0
{Journal}: CARBOHYDRATE RESEARCH
{Volume}: 368
{Pages}: 16-21
{ISBN/ISSN}: 0008-6215
{Keywords}: OYSTER MUSHROOM; ANTITUMOR POLYSACCHARIDES; EDIBLE MUSHROOM; ANTI-GLYCATION; EXTRACT; CULTIVATION; FUNGI; RATS; RIND; Pleurotus ostreatus; Mycelia; Heteroglycan; Structure; NMR studies; Antioxidant properties
{Abstract}: A heteroglycan (PS) isolated from the mycelia of Pleurotus ostreatus was found to consist of L-fucose, D-mannose, and D-glucose in a molar ratio of nearly 1:2:3. On the basis of acid hydrolysis, methylation analysis, periodate oxidation, and NMR studies (H-1, C-13, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, HMBC, and DEPT), the structure of the repeating unit of the PS was determined. The repeating unit had a branched backbone composed of (1 -> 6)-linked alpha and beta D-glucose and (1 -> 2)-linked alpha-L-fucose. Branching occurred at C-4 position of (1 -> 6)-linked alpha-D-glucopyranosyl residue with terminal beta-D-mannose and C-3 position of (1 -> 6)-linked beta-D-glucopyranosyl residue with (1 -> 6)-alpha-D-mannopyranosyl moiety terminated by alpha-D-glucose. The structure of the repeating unit of the PS was proposed as:
[GRAPHICS]
The antioxidant properties of the PS were studied. The scavenging activity of hydroxyl free radicals whose EC50 value of PS at 943 mu g/mL was determined. Further, EC50 value of the PS at 53 mu g/mL was observed for scavenging activity of superoxide free radicals. Chelating effects (54.82%) of ferrous ions were observed at 1 mg/mL of the PS. (C) 2013 Elsevier Ltd. All rights reserved.
{Author Address}: Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India; Univ Calcutta, Dept Bot, Mol & Appl Mycol & Plant Pathol Lab, Calcutta 700019, W Bengal, India; Univ Calcutta, Dept Bot, Mol & Appl Mycol & Plant Pathol Lab, Calcutta 700019, W Bengal, India; Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: India; India; India

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