{Reference Type}: Journal Article
{Author}: He, Peixin; Geng, Lujing; Mao, Duobin; Wang, Zheng; Xu, Chunping
{Year}: 2013
{Title}: PRODUCTION, PRELIMINARY CHARACTERIZATION, AND BIOACTIVITIES OF EXOPOLYSACCHARIDES FROM Pleurotus geesteranus 5 #
{Tag}: 0
{Star}: 0
{Journal}: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
{Volume}: 43
{Issue}: 1
{Pages}: 108-122
{ISBN/ISSN}: 1082-6068
{Keywords}: POLYSACCHARIDE-PROTEIN COMPLEXES; WATER-SOLUBLE POLYSACCHARIDES; SUBMERGED CULTURE-CONDITIONS; EXO-BIOPOLYMER PRODUCTION; ANTIOXIDANT ACTIVITIES; DIFFERENT FRACTIONS; ANTITUMOR-ACTIVITY; LIGHT-SCATTERING; TUBER-REGIUM; OPTIMIZATION; antioxidant; chromatography; exopolysaccharides; fermentation; Pleurotus geesteranus; purification; taguchi method
{Abstract}: The optimal culture conditions of exopolysaccharides (EPS) production in submerged culture medium by Pleurotus geesteranus 5 # were determined using an orthogonal matrix method. The optimal defined medium (per liter) was 60.0 g maltose, 5.0 g tryptone, 1 mM NaCl, 5 mM KH2PO4, and initial pH 6.0 at 28 degrees C. In the optimal culture medium, the maximum EPS production was 16.97 g/L in a shake flask. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by size exclusion chromatography (SEC), and their molecular characteristics were examined by a multiangle laser-light scattering (MALLS) and refractive index (RI) detector system. The approximate weight-average molar masses of the Fr-I and Fr-II of EPS were determined to be 3.263 x 104 and 5.738 x 103 g/mol, respectively. The low values of polydispersity ratio (1.176 and 1.124 for Fr-I and Fr-II, respectively) of EPSs mean that these EPS molecules exist much less dispersed in aqueous solution without forming large aggregates. Furthermore, the experiments in vitro indicated that P. geesteranus 5# EPS exhibit high antitumor and antioxidative effects.
{Author Address}: Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Henan, Peoples R China; Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Henan, Peoples R China; Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Henan, Peoples R China; Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Henan, Peoples R China; Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Henan, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China

{Reference Type}: Journal Article
{Author}: Mishra, K. K.; Pal, R. S.; ArunKumar, R.; Chandrashekara, C.; Jain, S. K.; Bhatt, J. C.
{Year}: 2013
{Title}: Antioxidant properties of different edible mushroom species and increased bioconversion efficiency of Pleurotus eryngii using locally available casing materials
{URL}: http://www.sciencedirect.com/science/article/pii/S0308814612018730
{Tag}: 5
{Star}: 0
{Journal}: Food Chemistry
{Volume}: 138
{Issue}: 2–3
{Pages}: 1557-1563
{Date Displayed}: 2013/6/1/
{Alternate Title}: Food Chemistry
{ISBN/ISSN}: 0308-8146
{Keywords}: Edible mushrooms; Antioxidant properties; Principal component analysis; Pleurotus eryngii; Casing materials; Biological efficiency
{Abstract}: Total phenolics, radical scavenging activity (RSA) on DPPH, ascorbic acid content and chelating activity on Fe2+ of Pleurotus citrinopileatus, Pleurotus djamor, Pleurotus eryngii, Pleurotus flabellatus, Pleurotus florida, Pleurotus ostreatus, Pleurotus sajor-caju and Hypsizygus ulmarius have been evaluated. The assayed mushrooms contained 3.94–21.67 mg TAE of phenolics, 13.63–69.67% DPPH scavenging activity, 3.76–6.76 mg ascorbic acid and 60.25–82.7% chelating activity. Principal Component Analysis (PCA) revealed that significantly higher total phenolics, RSA on DPPH and growth/day was present in P. eryngii whereas P. citrinopileatus showed higher ascorbic acid and chelating activity. Agglomerative hierarchical clustering analysis revealed that studied mushroom species fall into two clusters; Cluster I included P. djamor, P. eryngii and P. flabellatus, while Cluster II included H. ulmarius, P. sajor-caju, P. citrinopileatus, P. ostreatus and P. florida. Enhanced yield of P. eryngii was achieved on spent compost casing material. Use of casing materials enhanced yield by 21–107% over non-cased substrate.

{Reference Type}: Journal Article
{Author}: Mishra, K. K.; Pal, R. S.; Arunkumar, R.; Chandrashekara, C.; Jain, S. K.; Bhatt, J. C.
{Year}: 2013
{Title}: Antioxidant properties of different edible mushroom species and increased bioconversion efficiency of Pleurotus eryngii using locally available casing materials
{Tag}: 0
{Star}: 0
{Place Published}: Langford Lane, Kidlington, Oxford, OX5 1GB, United Kingdom
{Journal}: Food Chemistry
{Volume}: 138
{Issue}: 2-3
{Pages}: 1557-1563
{Date Displayed}: 2013
{ISBN/ISSN}: 03088146
{Original Publication}: Elsevier Ltd
{Keywords}: Phenols; Ascorbic acid; Bioconversion; Chelation; Composting; Fungi; Principal component analysis
{Abstract}: Total phenolics, radical scavenging activity (RSA) on DPPH, ascorbic acid content and chelating activity on Fe2+ of Pleurotus citrinopileatus, Pleurotus djamor, Pleurotus eryngii, Pleurotus flabellatus, Pleurotus florida, Pleurotus ostreatus, Pleurotus sajor-caju and Hypsizygus ulmarius have been evaluated. The assayed mushrooms contained 3.94-21.67 mg TAE of phenolics, 13.63-69.67% DPPH scavenging activity, 3.76-6.76 mg ascorbic acid and 60.25-82.7% chelating activity. Principal Component Analysis (PCA) revealed that significantly higher total phenolics, RSA on DPPH and growth/day was present in P. eryngii whereas P. citrinopileatus showed higher ascorbic acid and chelating activity. Agglomerative hierarchical clustering analysis revealed that studied mushroom species fall into two clusters; Cluster I included P. djamor, P. eryngii and P. flabellatus, while Cluster II included H. ulmarius, P. sajor-caju, P. citrinopileatus, P. ostreatus and P. florida. Enhanced yield of P. eryngii was achieved on spent compost casing material. Use of casing materials enhanced yield by 21-107% over non-cased substrate.   2012 Elsevier Ltd. All rights reserved.
{Notes}: Compilation and indexing terms, Copyright 2013 Elsevier Inc.
20130816036507
Antioxidant properties
Biological efficiency
Casing materials
Edible mushroom
Pleurotus eryngii
{Author Address}: Crop Protection Section, Vivekananda Institute of Hill Agriculture (Indian Council of Agricultural Research), Almora 263 601, Uttarakhand, India